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Invitrogen™ Qubit™ Flex Fluorometer
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Sample Volume: 1 to 20 µL
Excitation Wavelength:
Blue LED (max 460–480 nm)
Red LED (max 620–640 nm)
Specifications:
| Application | molecular biology | Warranty | 1 Year |
| Storage Temperature | Room Temperature | ||
| Product Type | Laboratory Equipment | ||
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Qubit™ Flex Fluorometer is an advanced benchtop device designed for the quantification of DNA, RNA, microRNA, and protein. With the capability to measure up to eight samples simultaneously, it offers improved throughput, accuracy, and reproducibility compared to traditional single-sample fluorometers. The Qubit Flex Fluorometer integrates seamlessly with Qubit reagents, enabling a highly optimized workflow for molecular biology applications.
Key Features:
| Feature | Description |
|---|---|
| Throughput | Measures up to 8 samples simultaneously, reducing assay time by 50%. |
| Accuracy | High sensitivity for low-concentration samples; avoids interference from non-target molecules. |
| Sample Volume | Requires only 1–20 μL per sample. |
| Data Export | Includes WiFi dongle, USB drive support, and direct computer connectivity for flexible data transfer. |
| User Interface | 8-inch color touchscreen with intuitive navigation and multi-language support (English, French, Spanish, Italian, German, simplified Chinese, Japanese). |
| On-Board Calculators | Integrated tools for reagent selection, working solution preparation, and molarity calculations. |
| Verification Program | Pre-loaded performance verification test ensures instrument reliability. |
| SmartStart Digital Experience | Includes interactive, visual guides for instrument installation, operation, and maintenance. |
| Extended Warranty | Comes with a three-year warranty, including factory-certified replacements for repairs. |
Advantages Over Traditional UV Spectrophotometry:
| Aspect | Qubit Flex Fluorometer | UV Spectrophotometry |
|---|---|---|
| Sensitivity | High; detects low-concentration DNA, RNA, and proteins. | Low; struggles with dilute samples. |
| Specificity | Fluorescent dyes bind only to target molecules. | Measures all molecules absorbing at 260 nm. |
| Throughput | Measures up to 8 samples simultaneously. | Single sample at a time. |
| Interference from Contaminants | Minimal; fluorescence only when bound to targets. | Significant; contaminants affect readings. |
| Ease of Use | Intuitive touchscreen and preloaded calculators. | Manual calibration and calculations. |
Applications:
| Application | Use Case |
|---|---|
| DNA Quantification | Preparation of NGS libraries, plasmids, PCR products, and genomic DNA. |
| RNA Quantification | Measuring total RNA, mRNA, and microRNA for transcriptomics. |
| Protein Quantification | Analysis of purified proteins, lysates, serum, and plasma samples. |
| MicroRNA Quantification | Determining concentrations of microRNA for regulatory and therapeutic studies. |
Specifications:
| Specification | Details |
|---|---|
| Sample Volume | 1–20 μL |
| Excitation Wavelength | 460–480 nm (Blue LED), 620–640 nm (Red LED) |
| Product Line | Qubit |
| Type | Fluorometer |
| Connectivity | WiFi Dongle, USB drive, or direct USB connection |
| Shipping Condition | Room temperature |
| Supported Languages | English, French, Spanish, Italian, German, simplified Chinese, Japanese |
| Warranty | Three years, including factory-certified refurbished replacement for repairs |
| For Use With Applications | DNA, RNA, microRNA, and protein quantification. |
Comparison: Qubit Flex Fluorometer vs Other Qubit Models and Other Fluorometers
| Feature | Qubit Flex Fluorometer | Qubit 4 Fluorometer | Thermo Scientific NanoDrop™ One | Promega Quantus™ Fluorometer |
|---|---|---|---|---|
| Sample Capacity | Up to 8 samples simultaneously | Single sample | Single sample | Single sample |
| Throughput | High throughput (up to 50% faster) | Standard throughput | Standard throughput | Standard throughput |
| Assay Compatibility | DNA, RNA, microRNA, protein | DNA, RNA, protein | DNA, RNA, protein | DNA, RNA |
| Accuracy | High accuracy with 1–20 µL, fluorescence-based | High accuracy with 1–20 µL, fluorescence-based | Moderate accuracy, UV absorbance detects all molecules at 260 nm | High accuracy, fluorescence-based |
| Optimized Reagents | Designed specifically for Qubit assay kits for reliable results | Designed for Qubit assay kits for reliable results | Compatible with nucleic acid quantitation without reagents | Compatible with fluorescence dye kits (e.g., Quant-iT) |
| Time to Results | <5 seconds per sample, simultaneous measurement reduces time | <3 seconds per sample | <10 seconds per sample | <5 seconds per sample |
| Data Export Options | Wi-Fi, USB drive, USB cable | Wi-Fi, USB drive, USB cable | USB, Ethernet, touchscreen display | USB |
| Screen | 8-inch color touchscreen | 5.7-inch color touchscreen | 7-inch color touchscreen | Small monochrome display |
| Special Features | Built-in molarity calculator; simultaneous measurement reduces variability | Preloaded RNA integrity assay, reagent calculator | Built-in contaminant analysis for UV spectra | Simple, budget-friendly option |
| Portability | Benchtop, not portable | Portable benchtop | Portable benchtop | Portable benchtop |
| Application Suitability | Best for high-throughput labs with diverse sample types | Best for low-to-moderate throughput labs | Routine nucleic acid quantification; less suited for low concentrations | Best for low-volume, high-accuracy applications |
| Consumables | Requires Qubit Flex reagents, assay kits, and assay tubes | Requires Qubit reagents, assay kits, and assay tubes | No proprietary reagents required, works with standard nucleic acid samples | Requires fluorescence dye kits |
Workflow and Operation:
- Prepare Assay Reagents:
- Use the on-board calculator to determine the reagent volumes required for your assay.
- Load Samples:
- Pipette 1–20 μL of sample into the appropriate assay tubes and mix with the working solution.
- Run Assay:
- Place up to 8 samples into the Qubit Flex Fluorometer and start the assay using the touchscreen interface.
- Review Results:
- Results are displayed in seconds, with options to export data via USB, WiFi, or direct cable.
- Post-Run Maintenance:
- Perform verification tests using preloaded programs to ensure consistent performance.
Package Includes:
| Component | Details |
|---|---|
| Qubit™ Flex Fluorometer | Includes 8-sample measurement capability. |
| Qubit™ Flex Power Cord | Required for instrument operation. |
| Wi-Fi Dongle | For cloud-based data export. |
Benefits:
- Throughput: Process multiple samples faster, reducing overall workflow time.
- Accuracy: Avoids contamination interference for highly reliable results.
- Ease of Use: Simplified operation with digital tools and an intuitive touchscreen.
- Cost-Effective: Reduces reagent waste with precise volume requirements.
For researchers in genomics, transcriptomics, and proteomics, the Qubit Flex Fluorometer offers an efficient and reliable solution for accurate quantification.
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