Invitrogen™ Qubit™ RNA High Sensitivity (HS) Assay Kits

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Quantification Range: 4 to 200 ng

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Specifications:

Application	Fluorescence-Based Assay
Storage Temperature	2-8°C
Product Type	Assay Kits	Forms	Liquid
Product Brand	Thermo Fisher Scientific™, Invitrogen
Product Grade	Molecular Biology

Overview
Resource

The Invitrogen™ Qubit™ RNA High Sensitivity (HS) Assay Kit is a highly sensitive fluorescence-based assay designed for precise quantification of low-abundance RNA samples. Optimized for use with Qubit Fluorometers, this assay provides superior accuracy and selectivity compared to absorbance-based methods, ensuring reliable measurement of RNA concentrations even at very low levels.

The assay uses RNA-selective fluorescent dyes that bind specifically to RNA, minimizing interference from DNA, proteins, free nucleotides, and common contaminants. This ensures highly accurate quantification critical for sensitive applications such as RNA sequencing (RNA-seq), transcriptomics, gene expression analysis, and next-generation sequencing workflows.

The Qubit RNA HS Assay Kit is ideal for laboratories working with limited RNA samples, precious clinical specimens, or low-yield RNA extractions requiring precise quantification.

Key Features

Ultra-High Sensitivity for Low-Concentration RNA

Quantifies RNA concentrations from 4 ng to 200 ng
Ideal for low-abundance RNA samples
Perfect for sensitive genomics and transcriptomics workflows

Highly Selective RNA Detection

Specifically detects RNA with minimal interference from:
- DNA
- Proteins
- Free nucleotides
- Common contaminants
Ensures accurate and reliable quantification

Superior Accuracy Compared to UV-Based Methods

Fluorescence-based detection improves specificity
Eliminates overestimation caused by contaminants
Provides reliable measurements for downstream applications

Fast and Easy Workflow

Requires only 1–20 μL sample volume
Simple assay setup with ready-to-use reagents
Results available within minutes using Qubit Fluorometer

Compatible with Multiple Qubit Fluorometers

Works with Qubit 1.0, 2.0, 3, 4, and Flex Fluorometers
Supports both low-throughput and high-throughput workflows

Applications

The Qubit RNA HS Assay Kit is ideal for:

RNA sequencing (RNA-seq) workflows
Transcriptomics and gene expression analysis
Next-generation sequencing (NGS) library preparation
Quantification of low-yield RNA samples
Molecular biology research
PCR and RT-PCR workflows
RNA extraction and purification workflows

Kit Contents and Storage

Component	Function	Storage Condition
RNA HS Assay Reagent	RNA-selective fluorescent dye	Room temperature
RNA HS Assay Buffer	Assay preparation buffer	Room temperature
RNA Standards	Calibration standards	Room temperature

Provides sufficient reagents for 100 or 500 assays.

Technical Specifications

Parameter	Specification
Assay Type	RNA High Sensitivity Quantification
Quantitation Range	4–200 ng
Detection Method	Fluorescence
Sample Volume	1–20 μL
Compatible Instruments	Qubit Fluorometers
Assays per Kit	100 or 500
Product Line	Quant-iT / Qubit
Shipping Condition	Room temperature
Unit Size	Each

Advantages for Genomics and Molecular Biology Laboratories

Highly sensitive RNA quantification for low-concentration samples
Essential for RNA-seq and transcriptomics workflows
Superior accuracy compared to UV spectrophotometry
Fast, simple, and reliable workflow
Compatible with Qubit Fluorometers used in sequencing laboratories
Ideal for genomics, biotechnology, and research laboratories

The Invitrogen™ Qubit™ RNA High Sensitivity (HS) Assay Kit provides a fast, accurate, and highly sensitive solution for quantifying low-abundance RNA samples. With its fluorescence-based detection, exceptional sensitivity, and compatibility with Qubit Fluorometers, this kit is an essential tool for RNA sequencing, transcriptomics, and molecular biology workflows requiring precise RNA concentration measurement.