Invitrogen™ Qubit™ RNA IQ Assay Kit

The Invitrogen™ Qubit™ RNA IQ Assay Kit is an advanced fluorescence-based solution designed to assess RNA integrity and degradation rapidly and accurately. Unlike traditional RNA quantification assays that measure only RNA concentration, this assay evaluates RNA quality by distinguishing between intact RNA and degraded RNA fragments.

Using two highly selective fluorescent dyes—one specific for large, intact RNA and another for small, degraded RNA—the assay generates an RNA Integrity and Quality Number (RNA IQ#), providing a clear indication of RNA sample integrity. This score, ranging from 1 to 10, allows researchers to quickly determine whether RNA samples are suitable for downstream applications such as RNA sequencing (RNA-seq), transcriptomics, and gene expression analysis.

The Qubit RNA IQ Assay Kit offers a fast and reliable alternative to gel electrophoresis and microfluidic-based RNA integrity analysis, making it ideal for modern genomics laboratories requiring rapid RNA quality assessment.

Key Features

Rapid RNA Integrity and Degradation Assessment

• Provides RNA IQ number (1–10) indicating RNA integrity
• Differentiates intact RNA from degraded RNA
• Identifies suitability of RNA samples for downstream applications

Dual-Dye Fluorescence Technology

• Large RNA dye detects intact RNA (mRNA, rRNA, tRNA)
• Small RNA dye detects degraded RNA fragments
• Provides percentage distribution of intact vs degraded RNA

Fast and Simple Workflow

• Minimal sample preparation required
• Requires only 1–20 μL RNA sample
• Results available within minutes

Highly Accurate and Reliable

• Accurate even in presence of:
  • Salts
  • Proteins
  • RNA stabilization reagents
  • Common laboratory contaminants

Ideal Alternative to Traditional RNA Integrity Methods

• Faster than gel electrophoresis
• Simpler workflow than microfluidic-based systems (e.g., Bioanalyzer, TapeStation)
• Provides clear, quantitative RNA quality scores

Applications

The Qubit RNA IQ Assay Kit is ideal for:

• RNA sequencing (RNA-seq) workflows
• Transcriptomics and gene expression analysis
• RNA quality control before NGS library preparation
• RNA extraction and purification workflows
• Molecular biology research
• Biotechnology and genomics laboratories
• RNA quality verification before RT-PCR and qPCR

RNA IQ Number Interpretation

Higher RNA IQ numbers indicate higher RNA integrity and suitability for sequencing and transcriptomics workflows.

Kit Contents

• RNA IQ Assay Reagents (dual-dye system)
• RNA IQ Assay Buffer
• RNA IQ Standards for calibration
• Complete reagents for 75 or 275 assays

Technical Specifications

Advantages for Genomics and Sequencing Laboratories

• Ensures RNA quality before RNA-seq workflows
• Prevents sequencing failure due to degraded RNA
• Faster and easier than electrophoresis-based methods
• Provides reliable RNA integrity scoring
• Essential quality control step for transcriptomics workflows
• Ideal for Illumina sequencing sample preparation

Comparison with Traditional RNA Integrity Methods

The Invitrogen™ Qubit™ RNA IQ Assay Kit provides a fast, reliable, and accurate solution for assessing RNA integrity and degradation. By generating an RNA IQ number and identifying intact versus degraded RNA, this assay enables researchers to ensure sample quality before critical applications such as RNA sequencing and transcriptomics. It is an essential quality control tool for genomics laboratories, sequencing facilities, and molecular biology research environments.