Invitrogen™ Qubit™ RNA Broad Range (BR) Assay Kit

The Invitrogen™ Qubit™ RNA Broad Range (BR) Assay Kit is a highly accurate fluorescence-based assay designed for precise quantification of RNA across a broad concentration range. Optimized for use with Qubit Fluorometers, this assay provides reliable RNA measurements even in the presence of contaminants that can interfere with traditional absorbance-based methods.

Unlike UV spectrophotometry, which measures all nucleic acids and contaminants absorbing at 260 nm, the Qubit RNA BR Assay Kit uses highly selective fluorescent dyes that bind specifically to RNA. This ensures accurate quantification by minimizing interference from DNA, proteins, free nucleotides, salts, and solvents.

The assay is ideal for laboratories working with RNA sequencing (RNA-seq), gene expression analysis, and molecular biology workflows requiring precise RNA quantification.

Key Features

Broad RNA Quantification Range

• Quantifies RNA concentrations from 10 ng to 1,200 ng
• Suitable for moderate to high concentration RNA samples
• Ideal for a wide range of molecular biology and sequencing workflows

Highly Selective RNA Detection

• Specifically detects RNA with minimal interference from:
  • DNA
  • Proteins
  • Free nucleotides
  • Common contaminants
• Ensures highly accurate RNA measurement

Superior Accuracy Compared to UV Methods

• Fluorescence-based detection ensures precise quantification
• Eliminates inaccuracies caused by contaminants
• Ideal for sensitive downstream applications

Fast and Simple Workflow

• Requires only 1–20 μL sample volume
• Ready-to-use assay reagents simplify workflow
• Results available in minutes using Qubit Fluorometer

Reliable Performance in Complex Samples

• Tolerates contaminants such as:
  • Salts
  • Solvents
  • Detergents
• Provides consistent results across diverse sample types

Applications

The Qubit RNA BR Assay Kit is ideal for a wide range of molecular biology and genomics applications, including:

• RNA quantification for RNA sequencing (RNA-seq)
• Gene expression analysis workflows
• Next-generation sequencing (NGS) library preparation
• RNA extraction and purification workflows
• Molecular biology and biotechnology research
• Quantification of total RNA from biological samples
• Preparation of RNA samples for PCR and RT-PCR

Instrument Compatibility

Compatible with all Invitrogen Qubit Fluorometers:

• Qubit™ 1.0 Fluorometer
• Qubit™ 2.0 Fluorometer
• Qubit™ 3 Fluorometer
• Qubit™ 4 Fluorometer
• Qubit™ Flex Fluorometer

Kit Contents and Storage

Provides sufficient reagents for 100 or 500 assays.

Technical Specifications

Advantages for Genomics and Molecular Biology Laboratories

• Highly accurate RNA quantification for sequencing workflows
• Essential for RNA-seq and transcriptomics applications
• Superior accuracy compared to UV absorbance instruments
• Fast and easy workflow with minimal sample volume
• Reliable performance across a wide range of RNA concentrations
• Ideal for genomics, biotechnology, and research laboratories

The Invitrogen™ Qubit™ RNA Broad Range (BR) Assay Kit provides a fast, accurate, and reliable solution for RNA quantification in molecular biology and genomics workflows. Its fluorescence-based detection, high selectivity, and broad quantification range make it an essential tool for laboratories performing RNA sequencing, gene expression analysis, and next-generation sequencing library preparation.