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Invitrogen™ Qubit™ dsDNA High Sensitivity (HS) Assay Kit
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Excitation/Emission: 510/527 nm
Quantitation Range 0.1 - 120ng
Specifications:
| Application | Fluorescence-Based Assay | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Assay Kits | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Qubit™ dsDNA High Sensitivity (HS) Assay Kit provides highly accurate and sensitive fluorescence-based quantification of low-abundance double-stranded DNA (dsDNA). Designed specifically for use with Qubit Fluorometers, this assay enables precise measurement of DNA concentrations that are too low to be reliably detected by UV spectrophotometry.
Using highly selective fluorescent dyes that bind specifically to double-stranded DNA, the assay minimizes interference from RNA, proteins, free nucleotides, and other contaminants. This ensures reliable and accurate quantification critical for next-generation sequencing (NGS), PCR, cloning, and other molecular biology workflows.
The Qubit dsDNA HS Assay Kit is ideal for applications requiring accurate quantification of precious, low-concentration DNA samples, such as NGS library preparation and genomic DNA analysis.
Key Features
Ultra-High Sensitivity DNA Quantification
- Quantifies dsDNA from 0.1 ng to 120 ng
- Ideal for low-abundance DNA samples
- Perfect for sensitive genomics and sequencing workflows
Highly Selective for Double-Stranded DNA
-
Specifically detects dsDNA with minimal interference from:
- RNA
- Single-stranded DNA
- Proteins
- Free nucleotides
- Common contaminants
Superior Accuracy Compared to UV-Based Methods
- Fluorescence-based detection improves specificity
- Eliminates overestimation caused by contaminants
- Provides reliable quantification for critical applications
Fast and Simple Workflow
- Requires only 1–20 μL sample volume
- Easy assay setup with ready-to-use reagents
- Rapid results using Qubit Fluorometer
Reliable Performance in Complex Samples
- Tolerates contaminants such as salts, solvents, and detergents
- Ensures consistent and reproducible quantification
Applications
The Qubit dsDNA HS Assay Kit is ideal for:
- Next-generation sequencing (NGS) library preparation
- Illumina sequencing workflows
- PCR and qPCR sample preparation
- DNA extraction and purification workflows
- Molecular cloning and genomics research
- DNA quantification for low-concentration samples
- Biotechnology and life sciences research
Instrument Compatibility
Compatible with:
- Qubit™ 2.0 Fluorometer
- Qubit™ 3 Fluorometer
- Qubit™ 4 Fluorometer
- Qubit™ Flex Fluorometer
Kit Contents and Storage
| Component | Function | Storage Condition |
|---|---|---|
| dsDNA HS Assay Reagent | DNA-selective fluorescent dye | Store as recommended |
| dsDNA HS Assay Buffer | Assay preparation buffer | Store as recommended |
| DNA Standards | Calibration standards | Store as recommended |
Provides sufficient reagents for 100 or 500 assays.
Technical Specifications
| Parameter | Specification |
|---|---|
| Assay Type | dsDNA High Sensitivity Quantification |
| Quantitation Range | 0.1–120 ng |
| Detection Method | Fluorescence |
| Sample Volume | 1–20 μL |
| Compatible Instruments | Qubit Fluorometers |
| Assays per Kit | 100 or 500 |
| Product Line | Qubit |
| Shipping Condition | Wet Ice |
| Unit Size | Each |
Advantages for Genomics and Sequencing Laboratories
- Highly sensitive DNA quantification for low-concentration samples
- Essential for Illumina NGS library preparation workflows
- Superior accuracy compared to UV spectrophotometry
- Fast, simple, and reliable workflow
- Ideal for genomics, biotechnology, and research laboratories
- Ensures optimal DNA input for sequencing applications
The Invitrogen™ Qubit™ dsDNA High Sensitivity (HS) Assay Kit provides a fast, accurate, and highly sensitive solution for quantifying low-abundance double-stranded DNA. Its fluorescence-based detection, high specificity, and compatibility with Qubit Fluorometers make it an essential tool for genomics, next-generation sequencing, and molecular biology workflows requiring precise DNA quantification.
- Pack Size: 500 assays 100 assays