MyFi™ Mix is an advanced PCR mastermix designed for highly efficient amplification of complex, GC-rich, long, or inhibitor-containing templates. It combines MyTaq™ Hot-Start DNA Polymerase with a proprietary proofreading enzyme, creating a unique dual-enzyme system with enhanced target affinity, improved fidelity, and exceptional robustness.

Supplied as a ready-to-use 2× mastermix, MyFi™ Mix simplifies PCR setup while ensuring accuracy, speed, and reproducibility across a wide range of molecular biology applications.

Ordering Information

Robust Performance

A powerful buffer system and enzyme blend enable reliable amplification of difficult targets, including inhibitor-rich samples or templates that typically fail with standard Taq.

High Sensitivity

Enhanced target affinity and high processivity support amplification from low-copy DNA, improving detection and reproducibility in demanding assays.

High Efficiency (Up to 10 kb)

Supports amplification of long fragments (up to 10 kb) and works effectively with DNA from human, animal, plant, or complex environmental sources.

Superior Specificity

MyTaq™ HS antibody-mediated hot-start formulation prevents non-specific amplification, allowing room-temperature setup without primer-dimer formation.

High Accuracy

The integrated proofreading enzyme provides 3.5× higher fidelity than Taq polymerase, producing PCR products suitable for cloning and downstream applications.

Convenient Mastermix Format

All-in-one 2× mix containing polymerases, dNTPs, MgCl₂, and reaction enhancers—requiring only template, primers, and water.

Ideal for improving throughput, reducing errors, and enabling automation.

MyFi™ Mix is engineered to amplify challenging templates up to 10 kb with exceptional reliability. Its dual-enzyme formulation provides the combined benefits of hot-start specificity, enhanced processivity, tolerance to inhibitors, and proofreading fidelity.

The mix is optimized for room temperature assembly, eliminating non-specific amplification and providing consistent performance even in low-template copy assays. PCR products generated using MyFi™ Mix contain 3' A overhangs, making them fully compatible with TA cloning workflows.

Its unique buffer and enzyme formulation reduces the need for optimization while enhancing speed, reproducibility, and suitability for automated high-throughput platforms.

Applications

• Amplification of complex or GC-rich templates
• Long-range PCR (up to 10 kb)
• Low-copy target detection
• Robust PCR from inhibitor-rich samples
• High-fidelity PCR for cloning
• TA cloning workflows
• Automation-friendly PCR assays

MyFi™ Mix provides a powerful, high-fidelity, and convenient PCR solution for researchers working with challenging templates, low-copy targets, or applications requiring superior accuracy. Its hot-start polymerase, proofreading activity, inhibitor resistance, and ready-to-use mastermix format make it an excellent choice for molecular biology laboratories seeking consistent, reproducible, and high-performance PCR results.