Solis Hot Start HiFi DNA Polymerase Kit

The Solis Hot Start HiFi DNA Polymerase Kit is a versatile, high-fidelity PCR reagent kit optimized for applications requiring exceptional precision and robust performance. Incorporating a chemically modified polymerase with 3′→5′ exonuclease proofreading activity, this enzyme delivers >100× higher fidelity than Taq polymerase, ensuring accurate amplification for cloning, sequencing, and mutagenesis.

Its superior processivity allows long-range amplification up to 24 kb from lambda DNA and 12 kb from complex genomic DNA, while proprietary Stability TAG™ technology ensures enzyme stability at room temperature for up to one month, enabling ambient shipping and bench-top handling.

Key Features & Benefits

• High Fidelity Amplification – >100× higher accuracy than Taq polymerase, validated by SMRT sequencing.
• Long-Range PCR – Amplifies up to 24 kb (lambda DNA) or 12 kb (gDNA).
• GC-Rich Tolerance – Robust amplification of targets with 30–79% GC content when used with the included GC-rich Enhancer.
• Hot-Start Technology – Chemical modification prevents nonspecific priming; activation in 12–15 minutes.
• Multiplexing – Supports amplification of 1–18+ targets with uniform performance.
• Bisulfite Sequencing Ready – Efficiently incorporates dUTP, compatible with workflows requiring uracil-containing DNA (e.g., bisulfite-treated DNA, FFPE samples, carry-over prevention).
• Room Temperature Stability – Thanks to Stability TAG™, the enzyme remains stable for 1 month at RT; eco-friendly shipping without dry ice.
• Glycerol-Free Option – Available upon request for freeze-drying applications.

Applications

• High-fidelity PCR for sequencing, cloning, protein expression, and site-directed mutagenesis
• Long-range and challenging PCR workflows
• GC-rich DNA template amplification
• Bisulfite sequencing and analysis of damaged DNA (e.g., FFPE samples)
• Multiplex PCR assays requiring high precision

Kit Components

• Solis Hot Start HiFi DNA Polymerase (1 U/µL)
• 5× HiFi Reaction Buffer with 10 mM MgCl₂ (final 2 mM in 1× reaction)
• 10× GC-rich Enhancer for difficult templates

Product Specifications

Ordering Information

The Solis Hot Start HiFi DNA Polymerase Kit is a robust, high-precision solution for PCR applications that demand accuracy, long-range capability, and stability. With >100× fidelity over Taq, excellent tolerance for GC-rich templates, and room temperature stability for simplified logistics, it is ideally suited for cloning, sequencing, mutagenesis, bisulfite sequencing, and challenging PCR workflows.