Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase, Colorless
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Specifications:
Application | PCR | ||
Storage Temperature | -5 to -30°C | ||
Product Type | PCR Master Mix | Forms | Liquid |
Product Brand | Thermo Fisher Scientific™ | ||
Product Grade | Molecular Biology | ||
Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase is an advanced polymerase designed to enhance the efficiency, specificity, and sensitivity of PCR reactions. Available in both colorless and green formats, it employs antibody-based inhibition to prevent non-specific amplification and ensures high-quality results in a variety of applications.
Key Features
Enhanced PCR Performance
- Higher Specificity and Sensitivity:
- Antibody-based hot-start mechanism ensures DNA polymerase activity is inhibited at room temperature, reducing non-specific amplification.
- Better Yield:
- Achieves high yields of target amplicons, even from low-template DNA samples.
- Wider Amplicon Range:
- Capable of amplifying DNA fragments up to 6 kb in routine applications.
Optimized Workflow
- Convenient Setup:
- Reactions can be set up at room temperature without affecting specificity or yield.
- Pre-Optimized Buffer:
- Supplied with a 10X DreamTaq Buffer containing magnesium chloride, minimizing the need for reaction optimization.
Green Format Options:
- DreamTaq Green Buffer:
- Contains a density reagent and two tracking dyes for direct loading of PCR products onto agarose gels.
- Colorless Format:
- Ideal for applications requiring downstream fluorescence or absorbance detection.
Applications
- Routine PCR
- Genotyping
- Colony PCR
- Reverse Transcription PCR (RT-PCR)
- Generation of PCR Products for TA Cloning
Specifications
Property | Details |
---|---|
Enzyme Type | DreamTaq Hot Start DNA Polymerase |
Concentration | 5 U/μL |
Reaction Format | Separate Components |
Hot Start | Antibody-based Hot Start |
Overhang | 3'-A |
Reaction Speed | Standard |
Amplicon Size Range | Up to 6 kb |
Shipping Condition | Dry Ice |
Storage Temperature | -5 °C to -30 °C |
Buffer | 10X DreamTaq Buffer with MgCl₂ |
Buffer Options | Green or colorless formats |
GC-Rich PCR Performance | Moderate |
Formats Available | Colorless and Green |
Product Availability
Catalog Number | Color | Quantity |
---|---|---|
EP1701 | Colorless | 200 Units |
EP1702 | Colorless | 500 Units |
EP1703 | Colorless | 2500 Units |
Advantages
- High Productivity:
- Achieve reliable and reproducible results with minimal reaction setup effort.
- Versatility:
- Suitable for a wide range of PCR applications, from routine to more complex setups.
- Time-Saving:
- Direct gel loading with DreamTaq Green Buffer simplifies workflows.
- Stability:
- Robust enzyme performance across a variety of annealing temperatures and Mg²⁺ concentrations.
Safety and Handling
- Storage Conditions: Store at -5 °C to -30 °C for long-term stability.
- Shipping Conditions: Shipped on dry ice to maintain enzyme activity.
Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase offers the ideal combination of specificity, sensitivity, and convenience for researchers requiring high-quality PCR results in diverse applications.
Comparison: Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase vs. OneTaq® Quick-Load® 2X Master Mix with Standard Buffer
Feature | Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase | OneTaq® Quick-Load® 2X Master Mix with Standard Buffer |
---|---|---|
Type | DNA polymerase (separate components) | Ready-to-use master mix (pre-mixed with buffer and dNTPs) |
Format | Available in colorless or green buffer formats | Quick-Load format includes tracking dyes for direct gel loading |
Concentration | 5 U/μL | 2X master mix |
Reaction Setup | Requires manual addition of buffer, dNTPs, and other components | Ready-to-use mix simplifies setup |
Hot-Start Mechanism | Antibody-based inhibition at ambient temperatures | Chemically modified hot-start DNA polymerase |
Overhang | 3'-A | 3'-A |
Buffer Options | - 10X DreamTaq Buffer (colorless) | - Standard Buffer (included in the master mix) |
- 10X DreamTaq Green Buffer with dyes | ||
Applications | - Routine PCR | - Routine PCR |
- Genotyping | - Colony PCR | |
- Colony PCR | - Long-template PCR | |
- TA cloning | - Amplification of difficult (GC-rich) templates | |
- RT-PCR | - DNA sequencing preparations | |
Amplicon Length Range | Up to 6 kb | Up to 10 kb for genomic DNA; up to 40 kb for lambda DNA |
Temperature Range for Setup | Room temperature (no need for cold setup) | Room temperature (no need for cold setup) |
Reaction Optimization | Minimal optimization required due to pre-optimized buffer | Pre-optimized with standard buffer |
Direct Gel Loading | Yes, with DreamTaq Green Buffer | Yes, included in Quick-Load format |
Shipping Condition | Dry ice | Dry ice |
Storage Temperature | -5 °C to -30 °C | -20 °C |
Reaction Speed | Standard | Standard |
Certifications | None specified | ISO-certified manufacturing ensures high quality |
GC-Rich PCR Performance | Moderate | High |
Available Formats | - Colorless: 200, 500, 2500 Units | - 50 reactions (0.2 mL), 200 reactions (1 mL), 1,000 reactions (5 mL) |
- Green: 200, 500, 2500, and 4 × 2500 Units | ||
Best Use Cases | - General-purpose PCR applications | - General-purpose and GC-rich PCR |
- Applications requiring flexibility in reaction component setup | - Applications needing quick setup with minimal handling |
Key Differences
- Ease of Use:
- DreamTaq™: Requires manual addition of components, offering flexibility for customization.
- OneTaq® Quick-Load®: Ready-to-use master mix simplifies setup and minimizes error.
- Performance with GC-Rich Templates:
- DreamTaq™: Moderate performance for GC-rich templates.
- OneTaq® Quick-Load®: Designed for better performance with difficult templates.
- Amplicon Size Range:
- DreamTaq™: Up to 6 kb.
- OneTaq® Quick-Load®: Up to 10 kb for genomic DNA and up to 40 kb for lambda DNA.
- Direct Gel Loading:
- Both products offer this feature but through different buffer formulations.
- Applications:
- DreamTaq™: Focuses on basic PCR needs, TA cloning, and genotyping.
- OneTaq® Quick-Load®: Broader application range, including challenging templates.
Choose Thermo Scientific™ DreamTaq™ for basic PCR workflows requiring flexibility and OneTaq® Quick-Load® for streamlined workflows or challenging templates.
- Pack Size: 500 UNITS 25000 UNITS 200 UNITS