SoliSD™ Lyo-compatible Bsm DNA Polymerase Kit

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Specifications:

Application	Isothermal Amplification
Storage Temperature	-20°C
Product Type	PCR Reagent	Forms	Liquid
Product Brand	Solis Biodyne
Product Grade	Molecular Biology

Overview
Resource

The SoliSD™ Lyo-compatible Bsm DNA Polymerase Kit is a glycerol-free, highly stable DNA polymerase system designed for isothermal amplification workflows. Based on the large fragment of DNA polymerase from Bacillus smithii, it combines strong strand displacement activity with Stability TAG™ technology, providing room temperature stability for at least 1 month. This lyophilization-compatible format is ideal for developing portable, field-ready diagnostic assays.

The kit includes the proprietary SoliSD™ Supplement system, which enhances enzyme performance by resolving non-template control (NTC) issues, minimizing variability, and enabling reaction setup at room temperature.

Key Features & Benefits

Glycerol-Free – Fully compatible with lyophilization and freeze-drying workflows.
Room Temperature Stability – Stable for ≥1 month at 37°C, enabling ambient shipping and storage.
Fast Results – Amplification detectable in as little as 4 minutes.
No NTC Signal – Minimizes false positives for reliable results.
Robust Activity – Strong strand displacement for LAMP and related isothermal methods.
Flexible Temperature Range – Active at 51–62°C, optimum performance at 60°C.
Versatile Applications – Suitable for nucleic acid amplification in research and assay development.

Kit Components (5-vial format)

SoliSD™ Glycerol-Free Bsm DNA Polymerase (40 U/µL) – 1 vial
25× SoliSD™ Supplement – 1 vial
10× Isothermal Reaction Buffer – 1 vial
100 mM MgSO₄ – 1 vial
10× GC-rich Enhancer – 1 vial

Applications

LAMP (Loop-mediated Isothermal Amplification)
Rapid nucleic acid detection assays
Amplification of GC-rich DNA templates
Development of lyophilized or portable assay formats
Research in infectious disease detection and molecular diagnostics

Product Specifications

Attribute	Detail
Format	Glycerol-free, lyo-compatible kit
Polymerase Source	Large fragment DNA polymerase from Bacillus smithii
Activity	Strong strand displacement
Exonuclease Activity	None (no 5′→3′ or 3′→5′)
Temperature Range	51–62°C (optimum 60°C)
Detection Time	From 4 minutes
Stability	≥1 month at 37°C
Storage	–20°C (routine)
Shipping	Room temperature

Ordering Information

Catalog Number	Size	Reaction Capacity
32-22-0000S	Small	100 rxn
32-22-00250	Medium	250 rxn
32-22-01000	Large	1000 rxn
32-22-05000	Bulk	5000 rxn

The SoliSD™ Lyo-compatible Bsm DNA Polymerase Kit is an advanced, glycerol-free polymerase system tailored for isothermal amplification and lyophilized assay development. With strong strand displacement activity, fast time-to-result, and ambient stability, it is an ideal choice for LAMP workflows, field diagnostics, and molecular research requiring portable and robust solutions.