Invitrogen™ Collibri™ PCR-free ES DNA Library Prep Kit for Illumina™ Systems with UD Indexes

The Invitrogen™ Collibri™ PCR-free ES DNA Library Prep Kit for Illumina Systems with Unique Dual (UD) Indexes is a complete, high-accuracy next-generation sequencing (NGS) library preparation solution designed to generate uniquely indexed DNA libraries without PCR amplification. This PCR-free workflow eliminates amplification bias, preserves the original genomic representation, and significantly improves sequencing accuracy, making it ideal for high-precision genomics applications.

The kit integrates advanced enzymatic fragmentation, cleanup reagents, and Unique Dual (UD) indexed adapters, enabling rapid preparation of sequencing-ready libraries in approximately two hours. Unique dual indexing provides the highest level of multiplexing accuracy by minimizing index hopping and cross-contamination, ensuring reliable sample identification even in high-throughput sequencing environments.

The enzymatic fragmentation (ES) technology produces uniform fragment sizes and high library yield, enabling consistent sequencing performance across Illumina platforms including NovaSeq™, NextSeq™, MiSeq™, and HiSeq™ systems.

Key Features

PCR-free Workflow for Maximum Sequencing Accuracy

• Eliminates PCR amplification bias and preserves true genomic representation
• Reduces duplicate reads and improves variant detection accuracy
• Ideal for high-fidelity sequencing applications

Unique Dual (UD) Indexing for Superior Multiplexing Reliability

• Includes UD indexed adapters to eliminate index misassignment
• Provides maximum sample tracking accuracy in multiplex workflows
• Essential for high-throughput sequencing and sensitive applications

Fast and Efficient Workflow

• Complete PCR-free library preparation in approximately 2 hours
• Streamlined protocol improves laboratory efficiency
• Reduces hands-on time and workflow complexity

Advanced Enzymatic Fragmentation Technology

• Controlled enzymatic DNA fragmentation ensures consistent library quality
• Generates high library yield suitable for quantification and sequencing
• Provides reproducible and reliable sequencing results

Broad Sample Compatibility

• Supports intact genomic DNA inputs ≥100 ng
• Compatible with DNA amplicons >300 bp
• Suitable for DNA samples from all species

Automation and High-Throughput Compatibility

• Compatible with automated liquid handling systems
• Supports miniaturized workflows for reduced reagent consumption
• Ideal for sequencing core facilities and high-throughput laboratories

Visual Workflow Monitoring

• Integrated dyes provide visual confirmation of workflow progress
• Improves preparation accuracy and reduces workflow errors

Applications

The Collibri PCR-free ES DNA Library Prep Kit with UD Indexes is optimized for demanding genomics and sequencing applications, including:

• Whole genome sequencing (WGS)
• Genome resequencing
• De novo genome assembly
• Variant detection and SNP analysis
• Human genomics and precision medicine research
• Cancer genomics
• Microbial genome sequencing
• Population genomics and epidemiological surveillance

Illumina Platform Compatibility

Fully compatible with Illumina next-generation sequencing systems, including:

• NovaSeq™ 6000
• NextSeq™ 550
• MiSeq™
• iSeq™
• HiSeq™ X, 2500, 3000, and 4000

Available Kit Configurations

Kit Contents and Storage

Ships on dry ice to preserve reagent integrity.

Technical Specifications

Advantages for Sequencing Laboratories

• Eliminates PCR bias for highly accurate sequencing results
• Unique dual indexing prevents index hopping and improves data integrity
• Fast workflow enhances laboratory productivity
• High library yield ensures reliable sequencing performance
• Fully compatible with Illumina NovaSeq, NextSeq, MiSeq, and HiSeq platforms
• Ideal for genomics research, sequencing core facilities, and biotechnology laboratories

The Invitrogen™ Collibri™ PCR-free ES DNA Library Prep Kit for Illumina Systems with UD Indexes provides a complete, high-fidelity solution for generating uniquely indexed DNA libraries without PCR amplification. Its enzymatic fragmentation technology, integrated unique dual indexing, and rapid workflow make it an ideal choice for sequencing laboratories requiring maximum accuracy, reliable multiplexing, and high-quality Illumina sequencing results across diverse genomics applications.