Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit, 50 pg to 2 μg

The Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit is a highly sensitive, fluorescence-based DNA quantification solution designed for precise and accurate double-stranded DNA (dsDNA) measurements across a broad dynamic range (50 pg to 2 μg). The assay provides higher sensitivity than UV absorbance-based methods, ensuring reliable quantification even in low-concentration samples.

📌 Compatible with most microplate readers and fluorometers

📌 Linear over three orders of magnitude for reliable quantification

📌 Specific for dsDNA even in the presence of RNA and free nucleotides

Key Features & Benefits

1. Ultra-Sensitive dsDNA Quantification

✔ Detects as little as 0.25 pg/µL dsDNA – Ideal for low-concentration DNA samples.

✔ Three Orders of Magnitude Linearity – Provides reliable quantitation from 50 pg to 2 µg.

✔ More Sensitive Than UV Absorbance Readings – Reduces sample loss and enables precise measurements with limited DNA amounts.

2. High Specificity for dsDNA

✔ Selective for Double-Stranded DNA – Minimizes interference from RNA, ssDNA, and free nucleotides.

✔ Consistent Across Various DNA Sources – Works with genomic DNA, viral DNA, plasmid DNA, PCR amplicons, and microarray samples.

3. Simple & Fast Workflow

✔ Easy-to-Use, One-Step Protocol – Mix dye with sample, incubate for 5 minutes, and measure fluorescence.

✔ Compatible with 96-Well & 384-Well Formats – Suitable for high-throughput DNA quantitation.

✔ Works with Most Fluorescence Microplate Readers & Fluorometers – Excitation/Emission Maxima: 502 nm / 523 nm.

4. Flexible Kit Options for Different Workflows

✔ P7589 – 1 x 1 mL bottle (~200 assays, 2 mL reaction volume).

✔ P11496 – 10 x 100 µL tubes for smaller-scale applications.

Technical Specifications

Applications

🔹 PCR & qPCR Sample Quantitation – Ensures accurate template input for amplification reactions.

🔹 Microarray Sample Preparation – Helps normalize DNA concentrations before hybridization.

🔹 Genomic DNA Quantitation – Ideal for high-throughput DNA extraction workflows.

🔹 Viral DNA Quantification – Measures low-copy number viral genomes in research applications.

🔹 DNA Damage & Repair Assays – Monitors DNA degradation and stability.

🔹 Enzyme Activity & DNA-Binding Protein Studies – Measures dsDNA concentrations in biochemical assays.

Workflow & Protocol

1️⃣ Prepare the Working Solution:

• Dilute PicoGreen reagent 1:200 in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.5–8.0).

2️⃣ Add DNA Sample & Incubate:

• Mix equal volumes of sample and PicoGreen working solution.
• Incubate for 5 minutes at room temperature in the dark.

3️⃣ Measure Fluorescence:

• Detect fluorescence at Excitation 502 nm / Emission 523 nm using a fluorometer or microplate reader.

Ordering Information

📦 Shipping Conditions: Room Temperature

🧊 Storage Conditions: 2–8°C

Why Choose the Quant-iT™ PicoGreen™ dsDNA Assay Kit?

✅ High Sensitivity & Accuracy – Detects as little as 0.25 pg/µL dsDNA with a broad dynamic range.

✅ Easy & Fast Protocol – One-step assay with a 5-minute incubation.

✅ Compatible with Most Fluorescence Readers – Works with fluorometers & microplate readers.

✅ Specific for dsDNA – Minimal interference from RNA, ssDNA, and nucleotides.

✅ Flexible Format – Available in single-bottle or multiple-tube formats for different experimental needs.

The Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit is a powerful, high-sensitivity fluorescence-based DNA quantitation solution ideal for PCR, qPCR, sequencing, and genomic DNA quantification. Its broad dynamic range, simple workflow, and compatibility with fluorescence-based detection systems make it a top choice for researchers requiring accurate DNA measurements.

🔍 Perfect for: Molecular biology, genomics, biotechnology, forensic DNA analysis, and clinical research applications.