Invitrogen™ Collibri™ PCR-free ES DNA Library Prep Kit for Illumina™ Systems with CD Indexes

The Invitrogen™ Collibri™ PCR-free ES DNA Library Prep Kit for Illumina Systems with CD (Combinatorial Dual) Indexes is a complete, high-performance next-generation sequencing (NGS) library preparation solution designed to generate indexed DNA libraries without PCR amplification. By eliminating PCR, the kit preserves the original genomic representation, minimizes amplification bias, and reduces duplicate reads, ensuring superior sequencing accuracy and data quality.

This kit integrates enzymatic fragmentation, cleanup modules, and CD indexed adapters into a single workflow, enabling rapid preparation of multiplexed sequencing-ready libraries. The PCR-free protocol can be completed in approximately two hours, providing exceptional efficiency while maintaining high library yield and reproducibility.

The advanced enzymatic shearing (ES) technology ensures uniform DNA fragmentation, making this kit ideal for demanding Illumina sequencing applications such as whole genome sequencing, variant analysis, and de novo assembly.

Key Features

PCR-free Workflow for Maximum Data Integrity

• Eliminates PCR amplification bias and sequence distortion
• Reduces duplicate reads and improves sequencing accuracy
• Preserves true genomic complexity

Integrated Combinatorial Dual (CD) Indexing

• Includes CD indexed adapters for multiplex sequencing
• Enables simultaneous sequencing of multiple samples
• Provides efficient sample tracking and sequencing flexibility

Fast and Efficient Library Preparation

• Complete PCR-free workflow in approximately 2 hours
• Reduced hands-on time increases laboratory productivity
• Streamlined workflow simplifies NGS library preparation

Advanced Enzymatic Fragmentation Technology

• Controlled enzymatic DNA fragmentation ensures uniform fragment size
• Generates high-yield libraries suitable for multiple sequencing runs
• Provides consistent and reproducible performance

Broad Sample Compatibility

• Supports intact genomic DNA inputs ≥100 ng
• Compatible with DNA amplicons >300 bp
• Suitable for DNA samples from all species

Automation and High-Throughput Ready

• Compatible with automated liquid handling platforms
• Supports miniaturized workflows to reduce reagent costs
• Ideal for high-throughput sequencing laboratories

Visual Workflow Monitoring

• Integrated dyes provide visual feedback during preparation
• Improves workflow accuracy and reduces preparation errors

Applications

The Collibri PCR-free ES DNA Library Prep Kit with CD Indexes is optimized for high-accuracy genomics and sequencing applications, including:

• Whole genome sequencing (WGS)
• Genome resequencing
• De novo genome assembly
• Variant detection and SNP analysis
• Cancer genomics research
• Microbial genome sequencing
• Population genomics studies
• Precision medicine research

Illumina Platform Compatibility

Fully compatible with Illumina sequencing systems, including:

• NovaSeq™ 6000
• NextSeq™ 550
• MiSeq™
• iSeq™
• HiSeq™ X, 2500, 3000, and 4000

Kit Contents and Storage

Ships on dry ice to maintain reagent stability.

Technical Specifications

Advantages for Sequencing Laboratories

• Eliminates PCR bias for accurate sequencing results
• Integrated CD indexing supports multiplex sequencing workflows
• Fast workflow improves laboratory efficiency
• High yield and reproducibility across multiple sequencing runs
• Compatible with Illumina NovaSeq, NextSeq, MiSeq, and HiSeq platforms
• Ideal for genomics research, sequencing core facilities, and biotechnology laboratories

The Invitrogen™ Collibri™ PCR-free ES DNA Library Prep Kit for Illumina Systems with CD Indexes provides a comprehensive, efficient, and high-fidelity solution for generating multiplexed DNA libraries without PCR amplification. Its enzymatic fragmentation technology, integrated combinatorial dual indexing, and rapid workflow make it an excellent choice for genomics laboratories requiring precise, unbiased, and scalable library preparation for Illumina sequencing platforms.