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Invitrogen™ Collibri™ PCR-free ES DNA Library Prep Kit for Illumina™ Systems with UD Indexes
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Specifications:
| Application | Next Generation Sequencing | ||
| Storage Temperature | -20°C, 2-8°C | ||
| Product Type | NGS library Prep kits | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Collibri™ PCR-free ES DNA Library Prep Kit for Illumina Systems with Unique Dual (UD) Indexes is a complete, high-accuracy next-generation sequencing (NGS) library preparation solution designed to generate uniquely indexed DNA libraries without PCR amplification. This PCR-free workflow eliminates amplification bias, preserves the original genomic representation, and significantly improves sequencing accuracy, making it ideal for high-precision genomics applications.
The kit integrates advanced enzymatic fragmentation, cleanup reagents, and Unique Dual (UD) indexed adapters, enabling rapid preparation of sequencing-ready libraries in approximately two hours. Unique dual indexing provides the highest level of multiplexing accuracy by minimizing index hopping and cross-contamination, ensuring reliable sample identification even in high-throughput sequencing environments.
The enzymatic fragmentation (ES) technology produces uniform fragment sizes and high library yield, enabling consistent sequencing performance across Illumina platforms including NovaSeq™, NextSeq™, MiSeq™, and HiSeq™ systems.
Key Features
PCR-free Workflow for Maximum Sequencing Accuracy
- Eliminates PCR amplification bias and preserves true genomic representation
- Reduces duplicate reads and improves variant detection accuracy
- Ideal for high-fidelity sequencing applications
Unique Dual (UD) Indexing for Superior Multiplexing Reliability
- Includes UD indexed adapters to eliminate index misassignment
- Provides maximum sample tracking accuracy in multiplex workflows
- Essential for high-throughput sequencing and sensitive applications
Fast and Efficient Workflow
- Complete PCR-free library preparation in approximately 2 hours
- Streamlined protocol improves laboratory efficiency
- Reduces hands-on time and workflow complexity
Advanced Enzymatic Fragmentation Technology
- Controlled enzymatic DNA fragmentation ensures consistent library quality
- Generates high library yield suitable for quantification and sequencing
- Provides reproducible and reliable sequencing results
Broad Sample Compatibility
- Supports intact genomic DNA inputs ≥100 ng
- Compatible with DNA amplicons >300 bp
- Suitable for DNA samples from all species
Automation and High-Throughput Compatibility
- Compatible with automated liquid handling systems
- Supports miniaturized workflows for reduced reagent consumption
- Ideal for sequencing core facilities and high-throughput laboratories
Visual Workflow Monitoring
- Integrated dyes provide visual confirmation of workflow progress
- Improves preparation accuracy and reduces workflow errors
Applications
The Collibri PCR-free ES DNA Library Prep Kit with UD Indexes is optimized for demanding genomics and sequencing applications, including:
- Whole genome sequencing (WGS)
- Genome resequencing
- De novo genome assembly
- Variant detection and SNP analysis
- Human genomics and precision medicine research
- Cancer genomics
- Microbial genome sequencing
- Population genomics and epidemiological surveillance
Illumina Platform Compatibility
Fully compatible with Illumina next-generation sequencing systems, including:
- NovaSeq™ 6000
- NextSeq™ 550
- MiSeq™
- iSeq™
- HiSeq™ X, 2500, 3000, and 4000
Available Kit Configurations
| Catalog Number | Number of Reactions | UD Index Set | Index Range |
|---|---|---|---|
| A38602024 | 24 Preps | Set A | 1–24 |
| A43602024 | 24 Preps | Set B | 25–48 |
| A43603024 | 24 Preps | Set C | 49–72 |
| A43604024 | 24 Preps | Set D | 73–96 |
| A38608196 | 96 Preps | Sets A–D | 1–96 |
Kit Contents and Storage
| Component | Storage Condition |
|---|---|
| Collibri PCR-free ES DNA Library Prep Kit | –20°C |
| Collibri DNA Library Cleanup Kit | 4°C |
| Collibri DNA UD Indexes | –20°C |
Ships on dry ice to preserve reagent integrity.
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Type | PCR-free ES DNA Library Prep Kit with Unique Dual Indexes |
| Input DNA | ≥100 ng |
| Sample Type | DNA |
| Library Type | Fragment Library |
| Workflow Duration | Approximately 2 hours |
| Amplification | PCR-free |
| Index Type | Unique Dual (UD) Indexes |
| Fragmentation Method | Enzymatic fragmentation |
| Automation Compatibility | Yes |
| Sequencing Compatibility | Illumina sequencing platforms |
| Species Compatibility | All species |
| Applications | WGS, Resequencing, De Novo Assembly |
| Storage Conditions | –20°C and 4°C |
| Shipping Condition | Dry Ice |
Advantages for Sequencing Laboratories
- Eliminates PCR bias for highly accurate sequencing results
- Unique dual indexing prevents index hopping and improves data integrity
- Fast workflow enhances laboratory productivity
- High library yield ensures reliable sequencing performance
- Fully compatible with Illumina NovaSeq, NextSeq, MiSeq, and HiSeq platforms
- Ideal for genomics research, sequencing core facilities, and biotechnology laboratories
The Invitrogen™ Collibri™ PCR-free ES DNA Library Prep Kit for Illumina Systems with UD Indexes provides a complete, high-fidelity solution for generating uniquely indexed DNA libraries without PCR amplification. Its enzymatic fragmentation technology, integrated unique dual indexing, and rapid workflow make it an ideal choice for sequencing laboratories requiring maximum accuracy, reliable multiplexing, and high-quality Illumina sequencing results across diverse genomics applications.
- Reactions & UD Index Set: 24 Preps, Set A, 1-24 24 Preps, Set B, 25-48 24 Preps, Set C, 49-72 24 Preps, Set D, 73-96 96 Preps, Set A-D, 1-96