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Invitrogen™ Fluorescein Di-β-D-Galactopyranoside (FDG), 5mg
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Specifications:
| Application | Cell Analysis | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Molecular Biology Reagent | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | Formula | C₃₂H₃₂O₁₅ |
Highly sensitive fluorogenic β-galactosidase substrate for live-cell and in vitro applications
Invitrogen™ FDG is one of the most sensitive substrates for detecting β-galactosidase activity in both cell-based and biochemical assays. Non-fluorescent FDG is sequentially hydrolyzed by β-galactosidase—first to fluorescein monogalactoside (FMG), then to highly fluorescent fluorescein. This enzymatic conversion can be monitored by the increase in absorbance or fluorescence.
FDG is cell-permeant, making it ideal for live-cell reporter assays, flow cytometry, and fluorescence microscopy to measure LacZ expression. Its high sensitivity enables the detection of low β-galactosidase levels, even in single cells.
Key Features
- High Sensitivity – Detects very low β-galactosidase activity
- Bright Green Fluorescence – Excitation/emission maxima ~490/514 nm
- Cell-Permeant – Suitable for live-cell and fixed-cell assays
- Multiplex-Compatible – Works alongside other fluorophores in multi-color experiments
- Versatile Detection – Signal can be measured by fluorescence or absorbance
Applications
- LacZ reporter gene assays
- Live-cell β-galactosidase detection in flow cytometry
- Microscopy-based enzyme activity visualization
- Drug screening and gene expression studies
- Enzyme kinetics and biochemical assays
Specifications
| Parameter | Details |
|---|---|
| Catalog Number | F1179 |
| Chemical Name | Fluorescein Di-β-D-Galactopyranoside |
| Quantity | 5 mg |
| Substrate Type | β-galactosidase substrate |
| Excitation/Emission | ~490/514 nm |
| Cell Permeability | Yes |
| Detection Method | Fluorescence or absorbance |
| Form | Solid |
| Storage Conditions | –5°C to –30°C |
| Shipping Conditions | Room temperature |
| Regulatory Status | Research Use Only (RUO) |
Invitrogen™ FDG is a gold-standard fluorogenic β-galactosidase substrate that combines exceptional sensitivity, live-cell compatibility, and flexible detection methods. Its ability to produce bright, stable fluorescence makes it a preferred choice for LacZ reporter assays in molecular biology and cell biology research.