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Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 780
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Specifications:
| Application | Flow Cytometry, Cell Analysis | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Flow Cytometry Reagents | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Infrared-Excited Viability Dye for Discriminating Dead Cells in Flow Cytometry
Fixable Viability Dye eFluor™ 780 is a high-performance viability dye designed for irreversible labeling of dead cells prior to fixation, permeabilization, or intracellular staining. Unlike non-fixable dyes like 7-AAD or propidium iodide, this dye allows for robust and consistent exclusion of dead cells in multi-color flow cytometry, including protocols that involve intracellular antigen detection.
With red laser (633 nm) excitation and peak emission at 780 nm, Fixable Viability Dye eFluor™ 780 is ideal for use in advanced multicolor panels, offering minimal spectral spillover and compatibility with a wide range of fluorochromes.
Key Features and Benefits
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Fixable and Irreversible
Enables dead cell exclusion after fixation/permeabilization, making it ideal for intracellular staining workflows. -
Infrared Fluorescence
Excited by the 633 nm red laser, with emission at 780 nm—compatible with detectors such as APC-eFluor™ 780 or APC-Alexa Fluor™ 750. -
Minimal Spectral Overlap
Minimal compensation required; <5% spillover into PE-Cy7; optimized for multiparameter flow cytometry. -
Pre-Diluted and Convenient
Supplied in anhydrous DMSO as a ready-to-use solution. Stable for up to 20 freeze-thaw cycles. -
Multi-Species Compatibility
Suitable for use with cells from all species, including human and mouse models.
Applications
- Viability discrimination in flow cytometry
- Dead cell exclusion in fixed and permeabilized samples
- T cell, B cell, and other immune profiling workflows
- Multicolor intracellular staining panels
- Sample quality control in cytometric assays
Technical Specifications
| Property | Details |
|---|---|
| Product Name | Fixable Viability Dye eFluor™ 780 |
| Catalog Numbers | 65-0865-14 (100 tests), 65-0865-18 (500 tests) |
| Detection Method | Fluorescence (633 nm excitation / 780 nm emission) |
| Color | Infrared |
| Form | Pre-diluted solution in anhydrous DMSO |
| Reagent Type | Viability Dye |
| Instrument Compatibility | Flow cytometer with red (633 nm) laser |
| Storage Conditions | –70°C with desiccant; protect from light and moisture |
| Stability | Stable for up to 20 freeze-thaw cycles |
| Unit Size | Each (100 tests) |
| Product Line | eBioscience™ |
| Intended Use | For Research Use Only. Not for use in diagnostic procedures. |
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 780 provides a reliable, fixable solution for discriminating dead cells in flow cytometry, especially in protocols requiring fixation and intracellular staining. With its infrared fluorescence, compatibility with multicolor panels, and robust stability, it is an essential reagent for immune monitoring, T cell functional assays, and advanced cytometry applications.
- Pack Size: For 100 Tests for 500 Tests