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Thermo Scientific™ XapI (ApoI) (10 U/μL), 500 units
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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ XapI (ApoI) is a conventional restriction enzyme that recognizes the degenerate sequence 5′ R^AATTY 3′ / 3′ YTTAA^R 5′, producing predictable DNA fragments for cloning and genetic analysis. Optimized for use at 37 °C in Tango Buffer, it ensures consistent and reliable digestion performance across a range of DNA substrates.
As part of Thermo Scientific’s comprehensive collection of restriction endonucleases, XapI is validated within the Five Buffer System, with the universal Tango buffer supplied for efficient double digestions. All buffers contain premixed BSA, enhancing enzyme stability and minimizing the effects of contaminants that may be present in DNA preparations.
Key Features
- Precise digestion – recognizes and cleaves R^AATTY sequences.
- Five Buffer System – ensures optimal activity with color-coded convenience.
- Universal Tango buffer – simplifies double-digestion workflows.
- Stable formulation – premixed BSA improves enzyme stability and fidelity.
- Quality assurance – produced under stringent QC standards for reproducibility.
- Versatile use – compatible with a wide range of molecular biology workflows.
Applications
- Molecular cloning.
- Restriction site mapping.
- Genotyping.
- Southern blotting.
- Restriction fragment length polymorphism (RFLP).
- SNP analysis.
Technical Specifications
| Property | Value |
|---|---|
| Enzyme | XapI (ApoI) |
| Recognition Sequence | 5′ R^AATTY 3′ / 3′ YTTAA^R 5′ |
| Cleavage Product | Defined cleavage within degenerate recognition sequence |
| Isoschizomers | AcsI, ApoI |
| Optimal Reaction Temperature | 37 °C |
| Compatible Buffer | 10X Tango Buffer |
| Concentration | 10 U/µL |
| Quantity | 500 units |
| Methylation Sensitivity | Not Dam-, Dcm-, or CpG-sensitive |
| Heat Inactivation | Yes |
| Type IIS Restriction Enzyme | No |
| Research Category | Traditional cloning |
| Storage Temperature | –20 °C |
Available Format
| Catalog Number | Quantity | Concentration | Format |
|---|---|---|---|
| ER1381 | 500 units | 10 U/µL | Enzyme + Buffers |
Thermo Scientific™ XapI (ApoI) (10 U/µL) delivers reliable and accurate DNA cleavage at degenerate R^AATTY recognition sites, making it a valuable tool for cloning, mapping, and genotyping applications. With its optimized Tango buffer compatibility, premixed BSA stabilization, and reproducible performance, XapI supports efficient and flexible DNA analysis in modern molecular biology workflows.