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Invitrogen™ Anza™ 26 Eco32I (EcoRV), 3000 units
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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Invitrogen™ Anza™ 26 Eco32I is a restriction enzyme that recognizes the sequence 5′ GAT^ATC 3′ / 3′ CTA^TAG 5′, producing blunt ends. It is optimized to deliver complete digestion in 15 minutes at 37 °C, with the flexibility to run extended incubations of up to 16 hours without star activity.
Part of the Anza™ Restriction Enzyme Cloning System, Eco32I is formulated for superior convenience. All Anza restriction enzymes operate in the same universal buffer system, enabling simultaneous multi-enzyme digestions in a single reaction without sequential steps or DNA clean-up. For additional workflow simplification, Anza Red Buffer is included with density reagent and tracking dyes for direct gel loading.
Key Features
- Fast digestion – complete DNA digestion in 15 minutes at 37 °C.
- Flexible protocols – overnight digestions up to 16 hours without star activity.
- Universal buffer – all Anza restriction enzymes function in a single buffer system.
- Convenient Red Buffer – includes tracking dyes for direct gel loading.
- Seamless workflow integration – compatible with Anza DNA Modifying Enzymes (T4 DNA Ligase, Alkaline Phosphatase, T4 PNK, DNA Blunt End, DNA End Repair Kits).
- Reliable results – ensures consistency across all DNA types.
Applications
- Molecular cloning.
- Restriction site mapping.
- Genotyping and SNP analysis.
- Southern blotting.
- Restriction fragment length polymorphism (RFLP).
Technical Specifications
| Property | Value |
|---|---|
| Enzyme | Eco32I (isoschizomer: EcoRV) |
| Recognition Sequence | 5′ GAT^ATC 3′ / 3′ CTA^TAG 5′ |
| Cleavage Product | Blunt ends |
| Optimal Reaction Temperature | 37 °C |
| Digestion Time | 15 minutes |
| Long Incubation Stability | Up to 16 hours without star activity |
| Compatible Buffers | Anza 10X Buffer, Anza 10X Red Buffer |
| Methylation Sensitivity | Not Dam-, Dcm-, or CpG-sensitive |
| Heat Inactivation | No |
| Type IIS Restriction Enzyme | No |
| Research Category | Traditional cloning |
| Storage Temperature | –20 °C |
Available Format
| Catalog Number | Quantity | Format |
|---|---|---|
| IVGN0266 | 3,000 units | Enzyme + Buffers |
Contents:
- 150 µL Anza 26 Eco32I
- 500 µL Anza Buffer (10X)
- 500 µL Anza Red Buffer (10X)
Invitrogen™ Anza™ 26 Eco32I provides precision blunt-end cleavage with unmatched workflow convenience. With its universal buffer system, compatibility with other Anza restriction enzymes, and the flexibility of short or overnight digestion without star activity, it simplifies cloning experiments. Combined with the Anza family of DNA Modifying Enzymes, it delivers a complete, efficient, and reproducible cloning workflow for molecular biology laboratories.