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Thermo Scientific™ Eco32I (EcoRV), HC (50 U/μL), 10,000 units
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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Eco32I (EcoRV), High Concentration (HC), is a blunt-cutting restriction enzyme that recognizes the sequence 5′ GAT^ATC 3′ / 3′ CTA^TAG 5′. Optimized for activity at 37 °C in Buffer R, this enzyme offers the same reliability as standard Eco32I but at a higher concentration of 50 U/µL, providing greater flexibility for high-throughput or large-scale cloning workflows.
As part of Thermo Scientific’s extensive portfolio of conventional restriction endonucleases, Eco32I (EcoRV) is optimized within the Five Buffer System and is supplied with the universal Tango buffer for efficient double digestions. All buffers are premixed with BSA to enhance enzyme stability and neutralize contaminants, ensuring reproducibility across molecular biology applications.
Key Features
- High-concentration format – 50 U/µL for maximum flexibility and efficiency.
- Precise blunt-end cleavage – at GAT^ATC sites.
- Five Buffer System – color-coded for optimized performance.
- Universal Tango buffer – simplifies double-digestion workflows.
- Stabilized buffers – premixed BSA enhances reliability.
- Quality assurance – produced under stringent QC standards for consistency.
Applications
- Molecular cloning.
- Restriction site mapping.
- Genotyping.
- Southern blotting.
- Restriction fragment length polymorphism (RFLP).
- SNP analysis.
Technical Specifications
| Property | Value |
|---|---|
| Enzyme | Eco32I (EcoRV) |
| Recognition Sequence | 5′ GAT^ATC 3′ / 3′ CTA^TAG 5′ |
| Cleavage Product | Blunt ends |
| Isoschizomers | EcoRV |
| Optimal Reaction Temperature | 37 °C |
| Compatible Buffer | 10X Buffer R |
| Supplied Buffer | Universal Tango buffer (for double digestions) |
| Concentration | 50 U/µL |
| Quantity | 10,000 units |
| Methylation Sensitivity | Not Dam-, Dcm-, or CpG-sensitive |
| Heat Inactivation | Yes |
| Type IIS Restriction Enzyme | No |
| Research Category | Traditional cloning |
| Storage Temperature | –20 °C |
Available Format
| Catalog Number | Quantity | Concentration | Format |
|---|---|---|---|
| ER0303 | 10,000 units | 50 U/µL | Enzyme + Buffers |
Thermo Scientific™ Eco32I (EcoRV), HC (50 U/µL) combines the precision of EcoRV blunt-end cleavage with the convenience of a high-concentration format. With optimized buffer compatibility, stabilized formulations, and universal Tango buffer for double digestions, it delivers superior performance for demanding molecular cloning and DNA analysis workflows.