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Thermo Scientific™ Eco32I (EcoRV) (10 U/μL)
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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Eco32I (EcoRV) is a high-quality conventional restriction enzyme that recognizes the sequence 5′ GAT^ATC 3′ / 3′ CTA^TAG 5′, generating blunt ends. Optimized for use at 37 °C in Buffer R, Eco32I provides consistent performance in cloning and DNA analysis workflows.
Part of Thermo Scientific’s large collection of conventional restriction endonucleases, Eco32I is validated within the Five Buffer System. Each enzyme is optimized for one of the five buffers, with the universal Tango buffer provided for efficient double digestions. All buffers are premixed with BSA, ensuring enzyme stability and binding of contaminants for reliable performance.
Key Features
- High-quality performance – stringent QC and industry-leading enzyme manufacturing.
- Blunt-end cleavage – precise digestion at GAT^ATC sites.
- Five Buffer System – color-coded buffers for optimal activity and flexibility.
- Universal Tango buffer – simplifies double digestions.
- Stable formulation – premixed BSA enhances stability and consistency.
- Versatile selection – wide range of restriction specificities available.
Applications
- Molecular cloning.
- Restriction site mapping.
- Genotyping.
- Southern blotting.
- Restriction fragment length polymorphism (RFLP).
- SNP analysis.
Technical Specifications
| Property | Value |
|---|---|
| Enzyme | Eco32I (EcoRV) |
| Recognition Sequence | 5′ GAT^ATC 3′ / 3′ CTA^TAG 5′ |
| Cleavage Product | Blunt ends |
| Isoschizomers | EcoRV |
| Optimal Reaction Temperature | 37 °C |
| Compatible Buffer | 10X Buffer R (Five Buffer System) |
| Supplied Buffer | Universal Tango buffer (for double digestions) |
| Concentration | 10 U/µL |
| Quantity | 2,000 units (other pack sizes available) |
| Methylation Sensitivity | Not Dam-, Dcm-, or CpG-sensitive |
| Heat Inactivation | Yes |
| Type IIS Restriction Enzyme | No |
| Research Category | Traditional cloning |
| Storage Temperature | –20 °C |
Available Formats
| Catalog Number | Quantity | Concentration | Format |
|---|---|---|---|
| ER0301 | 2,000 units | 10 U/µL | Enzyme + Buffers |
| ER0302 | 5 × 2,000 units | 10 U/µL | Enzyme + Buffers |
| ER0305 | 4,000 units | 10 U/µL | Enzyme + Buffers |
Thermo Scientific™ Eco32I (EcoRV) (10 U/µL) delivers precise blunt-end cleavage for molecular biology applications. With its robust Five Buffer System, universal Tango buffer for double digestions, and stabilization by premixed BSA, it ensures reproducibility and flexibility in cloning and DNA analysis. Eco32I is a reliable choice for researchers seeking accurate restriction digestion in traditional cloning workflows.
- Pack Size: 4,000 Units 2,000 UNITS 5 x 2,000 UNITS