Applied Biosystems™ Yfiler™ Plus PCR Amplification Kit

The Applied Biosystems™ Yfiler™ Plus PCR Amplification Kit is an advanced 6-dye Y-STR multiplex system designed for high-resolution analysis of male-specific DNA in forensic samples. Optimized for challenging casework, this kit enables reliable detection of trace male DNA in complex mixtures, particularly in the presence of high levels of female DNA.

With enhanced marker coverage, including rapidly mutating Y-STRs, the Yfiler™ Plus kit delivers superior discrimination power and improved performance with degraded and inhibited samples. It is approved for use in laboratories contributing DNA profiles to the U.S. National DNA Index System (NDIS) CODIS database, making it a trusted solution for forensic applications worldwide.

Key Features

Enhanced Discrimination Power

• Amplifies 27 Y-STR loci (17 core + 10 additional markers)
• Includes rapidly mutating (RM) Y-STRs for improved differentiation of closely related males
• Provides the highest discrimination capacity among Y-STR kits

Optimized for Challenging Samples

• Highly effective for male–female and male–male mixtures
• Improved recovery from trace, degraded, and inhibitor-rich samples
• Ideal for complex forensic casework such as sexual assault evidence

Improved Data Quality & Accuracy

• Expanded allelic ladder and virtual bins for accurate genotyping
• Clean baseline profiles for simplified data interpretation
• Concordant with existing Y-STR haplotype databases

Streamlined & Efficient Workflow

• Faster thermal cycling reduces time-to-results
• Supports direct PCR amplification from:
  • Blood and buccal samples on treated paper
  • Swabs and untreated substrates (with Prep-n-Go Buffer)
• Reduces need for DNA extraction in certain workflows

Validated Forensic Solution

• Fully integrated into Applied Biosystems™ forensic DNA workflow
• Developed in accordance with global standards (e.g., SWGDAM, DAB)
• Approved for use in NDIS CODIS database laboratories

Applications

• Forensic DNA analysis of male-specific samples
• Sexual assault and mixed DNA sample analysis
• Paternal lineage and kinship analysis
• Human identification and databasing
• Complex casework involving trace male DNA

Technical Specifications

Kit Contents & Storage

• Yfiler™ Plus Master Mix (2 × 0.5 mL)
• Yfiler™ Plus Primer Set (2 × 0.25 mL, protect from light)
• Yfiler™ Plus Allelic Ladder (2 × 0.025 mL, protect from light)
• DNA Control 007 (0.05 mL)

Storage:

• Store at –5°C to –30°C upon receipt
• Store at 2–8°C after initial use

Ordering Information

The Applied Biosystems™ Yfiler™ Plus PCR Amplification Kit is a powerful and reliable solution for forensic laboratories requiring high-resolution Y-STR analysis. With its enhanced discrimination capacity, robust performance with challenging samples, and streamlined workflow, it enables accurate identification of male DNA even in complex mixtures. This kit is an essential tool for laboratories focused on precision, efficiency, and advanced forensic DNA analysis.