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Applied Biosystems™ GlobalFiler™ PCR Amplification Kit
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Specifications:
| Application | qPCR, Forensics Human identification | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | PCR Amplification Kit | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Applied Biosystems™ | ||
| Product Grade | Molecular Biology | ||
The Applied Biosystems™ GlobalFiler™ PCR Amplification Kit is a next-generation 6-dye, 24-locus STR multiplex system designed for high-performance forensic DNA analysis. Engineered for maximum compatibility with global DNA databases, this kit delivers superior discrimination power, high sensitivity, and rapid amplification, making it ideal for demanding forensic casework applications.
Optimized for challenging and degraded samples, the GlobalFiler™ kit integrates advanced dye chemistry, enhanced buffer systems, and mini-STR technology to ensure reliable and reproducible results. As part of a fully validated and integrated forensic workflow, it is supported by industry-leading instrumentation, software, and technical expertise from Applied Biosystems™.
Key Features
High Discrimination Power & Global Compatibility
- 24-locus STR multiplex system with 6-dye technology
- Includes all loci recommended by the CODIS Core Loci Working Group
- Compatible with major global DNA databases, including European markers
Optimized for Challenging Samples
- Incorporates 10 mini-STRs for improved performance with degraded DNA
- Enhanced buffer system for low-level and inhibited samples
- Expanded DNA input range for greater sensitivity
Advanced Data Quality & Interpretation
- Improved intracolor balance for easier mixture interpretation
- Clean baseline profiles enabled by advanced oligo synthesis and purification
- Expanded allelic ladder for accurate detection of rare alleles
Efficient & Time-Saving Workflow
- Reduced amplification time compared to previous-generation kits
- Single-tube multiplex reaction simplifies workflow
- Fully integrated with Applied Biosystems™ forensic systems
Enhanced Gender Determination
- Multiple gender markers, including Amelogenin and Y markers
- Accurate typing even for degraded or Y-negative samples
Applications
- Forensic DNA casework analysis
- Human identification and databasing
- Criminal investigations and legal evidence processing
- Analysis of degraded or trace DNA samples
- Mixed DNA sample interpretation
Technical Specifications
| Parameter | Description |
|---|---|
| Product Name | GlobalFiler™ PCR Amplification Kit |
| Brand | Applied Biosystems™ |
| Technology | STR (Short Tandem Repeat) Analysis |
| Detection Method | Laser-induced Fluorescence |
| Dye Set | FAM, VIC, NED, TAZ, SID, LIZ |
| No. of Loci | 24 loci (including CODIS core markers) |
| Polymerase | Platinum™ Taq DNA Polymerase |
| Reaction Volume | 25 μL |
| Sample Type | Genomic DNA, Forensic Samples |
| GC-Rich PCR Performance | High |
| Format | Tube |
| Storage Conditions | -15°C to -25°C (long-term), 2–8°C after opening |
| Shipping Condition | Dry Ice |
Ordering Information
| Catalog Number | Number of Reactions |
|---|---|
| 4476135 | 200 Reactions |
| 4482815 | 1000 Reactions |
Compatible Instruments
- Veriti™ Pro Thermal Cycler
- ProFlex™ Thermal Cycler
- 3500 / 3500xL Genetic Analyzer
- 3730 DNA Analyzer
- SeqStudio™ Series Genetic Analyzers
The GlobalFiler™ PCR Amplification Kit sets a new standard in forensic DNA analysis by combining high sensitivity, robust performance, and global database compatibility in a single, streamlined workflow. With its ability to generate reliable results from even the most challenging samples, it is an essential solution for forensic laboratories seeking accuracy, efficiency, and maximum information recovery in human identification applications.
- Reactions: 1000 rxn 200 rxn