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Applied Biosystems™ Quantifiler™ Trio DNA Quantification Kit, 400 Rxn
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Specifications:
| Application | Forensics Human identification | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | PCR Amplification Kit | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Applied Biosystems™ | ||
| Product Grade | Molecular Biology | ||
The Quantifiler™ Trio DNA Quantification Kit is a highly advanced real-time PCR (qPCR) solution designed for precise quantification and quality assessment of human and male DNA in forensic samples. This robust kit enables laboratories to simultaneously measure total human DNA, human male DNA, and DNA quality indicators in a single reaction, supporting informed decision-making for downstream STR analysis.
Optimized for use in forensic workflows, the Quantifiler™ Trio kit enhances sample screening efficiency, improves STR profiling success rates, and ensures accurate analysis even with low-level, degraded, or inhibitor-rich samples. It integrates seamlessly with the Applied Biosystems™ 7500 Real-Time PCR System and HID Real-Time PCR Analysis Software for a fully validated and automated workflow.
Key Features
Highly Accurate Quantification
- Determines total human DNA and male DNA simultaneously
- Detects male DNA in excess female background (ratios ≥1:4000)
- Supports informed selection of STR analysis strategy
High Sensitivity & Wide Dynamic Range
- Detection limit: <1 pg/μL
- Quantification range: 0.005 ng/μL to >50 ng/μL
- Suitable for trace, low-template, and high-concentration samples
Enhanced DNA Quality Assessment
- Dual autosomal targets (Small Autosomal & Large Autosomal) for degradation assessment
- Quality Index predicts STR amplification success
- Enables identification of degraded DNA and optimization of workflow
Inhibitor Detection & Workflow Optimization
- Detects presence of PCR inhibitors
- Guides decisions on additional purification or use of specialized STR kits (e.g., miniSTR, GlobalFiler™)
- Improves overall downstream STR success rates
Highly Specific & Informative
- Specific to human and higher primate DNA
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Applicable to a wide range of sample types:
- Blood, buccal swabs, semen, tissue, and forensic casework samples
- Supports databasing, casework, and paternity testing
Efficient & Integrated Workflow
- Dual quantification system reduces sample consumption
- Results generated in approximately 1 hour
- Fully compatible with automated liquid handling systems
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Optimized for integration with:
- AmpFLSTR™ STR kits
- HID Real-Time PCR Analysis Software v1.2
- Applied Biosystems™ 7500 Real-Time PCR System
Applications
- Forensic DNA quantification and quality assessment
- Casework sample screening and triaging
- Human identification and databasing
- Paternity testing workflows
- STR workflow optimization and kit selection
Technical Specifications
| Parameter | Description |
|---|---|
| Product Name | Quantifiler™ Trio DNA Quantification Kit |
| Brand | Applied Biosystems™ |
| Technology | Real-Time PCR (TaqMan® qPCR) |
| Targets | Small Autosomal (SA), Large Autosomal (LA), Y-chromosome |
| Sensitivity | <1 pg/μL |
| Quantification Range | 0.005 ng/μL to >50 ng/μL |
| Quantity | 400 reactions |
| Sample Type | Human DNA (forensic samples) |
| Workflow Time | ~1 hour |
| Compatibility | Applied Biosystems™ 7500 Real-Time PCR System |
| Software | HID Real-Time PCR Analysis Software v1.2 |
| Automation | Compatible with robotic liquid handling systems |
| Storage Conditions | -5°C to -30°C (long-term), 2–8°C after opening |
Unique Advantages
- Triple-target system provides both quantity and quality insights in a single assay
- Enables intelligent STR kit selection (autosomal, Y-STR, or miniSTR)
- Improves success rates for challenging forensic samples
- Reduces rework and increases laboratory efficiency
- Fully integrated into a validated forensic DNA workflow system
The Quantifiler™ Trio DNA Quantification Kit is an essential component of modern forensic DNA workflows, delivering accurate quantification, detailed quality assessment, and actionable insights in a single assay. By enabling laboratories to make informed decisions before STR amplification, it significantly enhances efficiency, reduces sample waste, and improves overall success rates. This kit is a critical tool for forensic laboratories seeking precision, reliability, and optimized DNA analysis workflows.