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Virplex® Viral Meningitis RealTime PCR Kit, 7-Plex, 48 Rxn
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Targets: HSV-1, HSV-2, VZV, Enteroviruses (EVs), Human Parechoviruses (HPeVs), West Nile Virus (WNV), Toscana Virus (TOSV).
Specifications:
| Application | qPCR, Molecular Diagnostics | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Lyophilized |
| Product Brand | VIRCELL | ||
| Product Grade | Molecular Biology | ||
The Virplex® Viral Meningitis RealTime PCR Kit is a multiplex real-time PCR assay for the one-step detection of nucleic acids from the main viral agents causing meningitis in human cerebrospinal fluid (CSF) samples. This CE-marked kit enables rapid, sensitive, and differential detection of seven clinically relevant viral pathogens, providing laboratories with a comprehensive molecular tool for viral meningitis diagnostics and outbreak investigations.
Key Features
- 8-target multiplex design – two reaction tubes per sample.
- Simultaneous analysis of 7 viruses – HSV-1, HSV-2, VZV, Enteroviruses (EVs), Human Parechoviruses (HPeVs), West Nile Virus (WNV), Toscana Virus (TOSV).
- Bacteriophage MS2 RNA internal control – monitors sample integrity, RNA quality, and inhibition.
- Compatible with 5-channel qPCR systems – FAM, HEX (VIC), Q705 (Cy5.5), Texas Red (ROX), Cy5.
- Fast turnaround – results in under 2 hours.
- Lyophilized reagents – master mix and positive control for long-term stability and reduced transport costs.
- Flexible formats – available as vials or pre-dispensed strip-divisible plates.
- CE-certified – validated for clinical molecular diagnostics.
Applications
- Detection of viral meningitis pathogens in CSF samples.
- Syndromic differential diagnosis of meningitis.
- Epidemiological surveillance of viral CNS infections.
- Validation and verification of molecular meningitis testing workflows.
- Outbreak response and clinical virology research.
Product Details
| Property | Value |
|---|---|
| Product Name | Virplex® Viral Meningitis RealTime PCR Kit |
| References | RTPCR011 (vial format), RTPCR011-LPD (pre-dispensed strips) |
| Nº Tests | 48 |
| Class | CE |
| Sample Type | Human cerebrospinal fluid (CSF) |
| Pathogens Detected | HSV-1, HSV-2, Varicella Zoster Virus (VZV), Enteroviruses (EVs), Human Parechoviruses (HPeVs), West Nile Virus (WNV), Toscana Virus (TOSV) |
| Internal Control | Bacteriophage MS2 RNA |
| PCR Cycler Compatibility | FAM, HEX (VIC), Texas Red (ROX), Q705 (Cy5.5), Cy5 |
| Time to Results | < 2 hours |
| Formats | Lyophilized vials or pre-dispensed 96 × 0.1 mL strip-divisible plate |
| Kit Components | Master Mix A & B, reconstitution solution, positive control, negative control |
Targets & Channels
| Target Pathogen | Gene Target | Channel |
|---|---|---|
| HSV-1 | US4 gene | FAM |
| HSV-2 | UL27 gene | HEX/VIC |
| Varicella Zoster Virus (VZV) | ORF29 gene | Texas Red/ROX |
| Enteroviruses (EVs) | 5’ UTR gene | FAM |
| Human Parechoviruses (HPeVs) | 5’ UTR gene | Texas Red/ROX |
| West Nile Virus (WNV) | 3’ UTR gene | Q705/Cy5.5 |
| Toscana Virus (TOSV) | N gene | HEX/VIC |
| Internal Control | Exogenous RNA (MS2 bacteriophage) | Cy5 |
Virplex® Meningitis RealTime PCR Kits – Comparison
| Feature | Bacterial Meningitis Kit | Viral Meningitis Kit |
|---|---|---|
| Product Name | Virplex® Bacterial Meningitis RealTime PCR Kit | Virplex® Viral Meningitis RealTime PCR Kit |
| Reference | RTPCR014-LPD-R | RTPCR011 / RTPCR011-LPD |
| Nº Tests | 48 | 48 |
| Class | RUO (Research Use Only) | CE |
| Sample Type | Human cerebrospinal fluid (CSF) | Human cerebrospinal fluid (CSF) |
| Pathogens Detected | - Streptococcus pneumoniae (SPN) - Listeria monocytogenes (LMO) - Streptococcus agalactiae (SAG, GBS) - Haemophilus influenzae (HIN) - Neisseria meningitidis (NME) - Escherichia coli K1 (ECOK1) | - Herpes simplex virus 1 (HSV-1) - Herpes simplex virus 2 (HSV-2) - Varicella zoster virus (VZV) - Enteroviruses (EVs) - Human parechoviruses (HPeVs) - West Nile virus (WNV) - Toscana virus (TOSV) |
| Total Targets | 7 (6 pathogens + internal control) | 8 (7 pathogens + internal control) |
| Internal Control | DNA internal control | Bacteriophage MS2 RNA control |
| PCR Format | 2 tubes per sample | 2 tubes per sample |
| PCR Cycler Compatibility | 4-channel: FAM, HEX (VIC), Texas Red (ROX), Cy5 | 5-channel: FAM, HEX (VIC), Texas Red (ROX), Q705 (Cy5.5), Cy5 |
| Turnaround Time | < 2 hours | < 2 hours |
| Reagent Format | Lyophilized master mix & positive control | Lyophilized master mix A & B, positive/negative control |
| Kit Formats | Vials or pre-dispensed strip-divisible plate | Vials or pre-dispensed strip-divisible plate |
| Applications | Detection of major bacterial causes of meningitis in CSF samples | Detection of major viral causes of meningitis in CSF samples |
| Related AMPLIRUN® Controls | SPN, LMO, SAG, HIN, NME, ECOK1 DNA Controls | HSV-1, HSV-2, VZV, EVs, HPeVs, WNV, TOSV Controls + AMPLIRUN® Total Meningitis Control (CSF) |
- Virplex® Bacterial Meningitis RealTime PCR Kit – RTPCR014-LPD-R
- AMPLIRUN® Controls:
- AMPLIRUN® Total Meningitis Control (CSF)
The Virplex® Viral Meningitis RealTime PCR Kit delivers a rapid, accurate, and multiplex molecular solution for the detection of the most clinically relevant viral pathogens associated with meningitis. Its broad coverage, internal control, and lyophilized stability make it an indispensable tool for clinical virology laboratories, outbreak surveillance, and epidemiological research.
- Format: Lyophilized master mix, Vial Format Pre-dispensed 48 × 0.1 mL Tear-off 8-Tube Strip Mat with lyophilized master mix + caps.