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Invitrogen™ PureLink™ Pro 96 Viral RNA/DNA Purification Kit, 4 x 96 Preps
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Specifications:
| Application | DNA Extraction and Purification | ||
| Storage Temperature | Room Temperature, 2-8°C | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ Pro 96 Viral RNA/DNA Purification Kit is the only 96-well silica plate system capable of extracting both viral RNA and viral DNA from 200 μL of cell-free fluids such as plasma, serum, and cerebrospinal fluid. It provides reliable, high-throughput nucleic acid purification in as little as 30 minutes, without loss of sensitivity compared to traditional spin-column formats.
Optimized buffers containing guanidine salts and carrier RNA ensure maximum recovery and protection from RNase degradation, while the improved semi-skirted 96-well plate design prevents cross-contamination and ethanol carryover. The kit is compatible with centrifuges, vacuum manifolds, and robotic liquid handling systems, making it ideal for automated viral nucleic acid purification workflows.
Key Features
- Dual-Function Kit – Extracts both viral RNA and DNA from cell-free samples.
- High Sensitivity – Delivers consistent results equal to spin-column systems in qPCR and qRT-PCR.
- Fast & Efficient – Complete viral nucleic acid purification in ~30 minutes.
- Optimized Chemistry – Guanidine buffers enhance nucleic acid recovery, while carrier RNA protects RNA integrity.
- Flexible Processing – Supports centrifuge (≥2250 × g), benchtop vacuum, or automated platforms.
- Reliable Performance – No cross-contamination; consistent Ct values demonstrated in qRT-PCR.
Applications
- Extraction of viral RNA and DNA from plasma, serum, or cerebrospinal fluid.
- Preparation of viral nucleic acids for qPCR, qRT-PCR, cDNA library construction, NGS, and hybridization assays.
- Nucleic acid labeling and blotting applications (Northern, Southern).
- Research in virology, molecular diagnostics, and infectious disease surveillance.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Viral RNA/DNA Purification Kit (96-well) |
| Catalog Number | 12280096A (4 plates) |
| Isolation Technology | Silica 96-well plate |
| Sample Input | 200 μL cell-free fluid |
| Sample Types | Plasma, serum, cerebrospinal fluid, cell-free culture supernatants |
| Yield | >40 μg nucleic acids (binding capacity) |
| Elution Volume | 100–150 μL RNase-free water |
| Test Time | ~30 minutes |
| DNA/RNA Quality | Suitable for PCR, qPCR, RT-PCR, NGS, and hybridization |
| Throughput | High-throughput compatible (manual or automated) |
| Shipping Condition | Room temperature |
| Storage | Carrier RNA at –20 °C; Proteinase K at 4 °C; other components at RT |
| Unit Size | Each kit (4 plates) |
Kit Contents
- Carrier RNA (2.2 mg, store at –20 °C)
- Viral Lysis Buffer (L22)
- Wash Buffers II
- Proteinase K
- RNase-free Water
- 96-Well Viral Filter Plates
- 96 Deep-Well Blocks
- Foil Seals
Unique Advantages
- First dual RNA/DNA 96-well viral purification system.
- Ensures well-to-well consistency for high-throughput qPCR/qRT-PCR viral detection.
- Demonstrates scalability – same performance as spin-column kits in automated plate format.
- Compatible with major robotic systems including Biomek™, Eppendorf epMotion™, and TECAN Freedom Evo™.
The Invitrogen™ PureLink™ Pro 96 Viral RNA/DNA Purification Kit streamlines viral nucleic acid extraction, delivering sensitivity, reproducibility, and throughput without compromise. By enabling simultaneous purification of viral RNA and DNA in a high-throughput 96-well format, it supports demanding virology workflows for qPCR, sequencing, hybridization, and genomic studies.