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Invitrogen™ PureLink™ Viral RNA/DNA Mini Kit, for 50 preps
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Specifications:
| Application | Nucleic Acid Extraction | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Sample Prep | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ Viral RNA/DNA Mini Kit provides a rapid, reliable method for purifying viral RNA and DNA from various biological samples. This silica spin-column-based kit enables efficient isolation of high-quality viral nucleic acids in just 45 minutes, ideal for downstream applications like RT-PCR, qPCR, and NGS.
Key Features
- High Sensitivity:
- Elution volumes as low as 10–50 μL maximize nucleic acid concentration for sensitive downstream analysis.
- Versatile Sample Compatibility:
- Suitable for fresh or frozen cell-free biological fluids, including plasma, serum, cerebrospinal fluid, raw and stabilized saliva, nasopharyngeal viral transfer medium (NPVTM), and cell culture supernatants.
- Quick Workflow:
- Purification process completed in approximately 45 minutes per prep.
- Spin Column-Based Centrifugation:
- Avoids sample cross-contamination and ensures reproducible results.
- High Yield:
- Recover up to 5 μg of viral RNA/DNA per prep with excellent purity.
Specifications
| Feature | Details |
|---|---|
| Column Type | Spin Column with Silica Spin Column Technology |
| Sample Type | Cell-free fluids, cell culture supernatants, saliva, NPVTM |
| Elution Volume | Adjustable: 10–50 μL |
| Final Product | Viral RNA and DNA |
| Applications | RT-PCR, qPCR, cDNA library construction, NGS, blot hybridization |
| High-throughput Compatibility | Manual use only |
| Test Time | 45 minutes per prep |
| Yield | Up to 5 μg per prep |
| Starting Material | Up to 500 μL sample volume |
| Quantity | 50 preps per kit |
| Storage Conditions | Reagents stored at room temperature; Carrier RNA stored at –20°C |
| Shipping Condition | Room temperature |
Applications
- Viral RNA Detection:
- Efficiently extract RNA for RT-PCR, qPCR, and sequencing.
- Viral DNA Isolation:
- Extract DNA from viruses for genotyping or molecular analysis.
- Next-Generation Sequencing (NGS):
- High-quality RNA and DNA suitable for advanced sequencing workflows.
- cDNA Library Construction:
- Use purified RNA for high-fidelity cDNA synthesis.
- Hybridization and Blotting:
- Prepare nucleic acids for Northern and Southern blotting.
Advantages
| Feature | Benefit |
|---|---|
| Low Elution Volumes | High nucleic acid concentrations for sensitive downstream applications. |
| Wide Sample Compatibility | Flexible across a variety of sample types and volumes. |
| Fast Workflow | Complete purification in under an hour. |
| High Yield and Purity | Recover up to 5 μg of RNA/DNA with minimal contaminants. |
| Spin Column Technology | Avoids cross-contamination and ensures reproducibility. |
Kit Contents
| Component | Quantity | Storage Conditions |
|---|---|---|
| Carrier RNA | 310 μg | –20°C |
| Viral Lysis Buffer L22 | 32 mL | Room temperature |
| Wash Buffer WII | 15 mL | Room temperature |
| Proteinase K | 2 x 1.6 mL | Room temperature or 4°C |
| RNase-Free Water E3 | 15.5 mL | Room temperature |
| Viral Spin Columns with Collection Tubes | 50 columns | Room temperature |
| Wash Tubes | 2 x 50 | Room temperature |
| Recovery Tubes | 50 | Room temperature |
Comparison with Competitor Kits
| Feature | Invitrogen™ PureLink™ Viral RNA/DNA Mini Kit | Qiagen QIAamp Viral RNA Mini Kit | Zymo Quick-RNA Viral Kit |
|---|---|---|---|
| Target Molecules | RNA and DNA | RNA only | RNA and DNA |
| Sample Compatibility | Plasma, serum, CSF, saliva, NPVTM | Plasma, serum, CSF, swabs | Plasma, serum, CSF |
| Elution Volume | 10–50 μL | 50 μL | 6–50 μL |
| Yield per Prep | Up to 5 μg | Up to 8 μg | Up to 10 μg |
| Processing Time | ~45 minutes | ~50 minutes | ~30 minutes |
| High-Throughput Compatibility | No | No | Yes |
| Storage | Room temperature | Room temperature | Room temperature |
Suggested Pairing
- Downstream Amplification:
- Combine with Invitrogen™ SuperScript™ IV RT for RNA amplification.
- NGS Analysis:
- Use with Ion Torrent™ NGS platforms for sequencing workflows.
- Quantification:
- Pair with Thermo Scientific™ NanoDrop™ Spectrophotometer for precise nucleic acid quantification.
The Invitrogen™ PureLink™ Viral RNA/DNA Mini Kit provides reliable and efficient viral nucleic acid isolation, ensuring high yield, purity, and sensitivity for critical downstream molecular biology applications.
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