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Thermo Scientific™ Halt™ Phosphatase Inhibitor Single-Use Cocktail
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Specifications:
| Application | molecular biology, Protein Purification | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ Halt™ Phosphatase Inhibitor Single-Use Cocktail is a ready-to-use solution designed to preserve the phosphorylation state of proteins during and after cell lysis or tissue extraction. Supplied as a 100X concentrated liquid, this cocktail provides effective inhibition of both serine/threonine and tyrosine phosphatases, ensuring accurate analysis of phosphorylation-dependent cellular processes.
Optimized for convenience and performance, the single-use format minimizes contamination risk while enabling precise dosing for consistent experimental results.
Key Features
Comprehensive Phosphatase Inhibition
- Inhibits serine/threonine phosphatases and protein tyrosine phosphatases (PTPs)
- Preserves phosphorylation-dependent signaling pathways
- Ensures integrity of phosphoproteins during sample preparation
100X Concentrated Format
- Supplied as a 100X concentrate for dilution to 1X working concentration
- Adjustable concentration (1X–3X) for samples with high phosphatase activity
- Enables flexibility across different sample types
Single-Use Convenience
- Pre-aliquoted formats reduce contamination risk
- Ensures reproducibility across experiments
- Ready-to-use liquid formulation for rapid integration
Broad Compatibility
- Compatible with detergent-based lysis buffers
- Works with BCA and Bradford (Coomassie) protein assays
- Suitable for immunoprecipitation and kinase assays
Dialyzable Formulation
- Inhibitors can be removed by dialysis or desalting if required
- Suitable for workflows involving downstream incompatibilities
Optimized Storage
- Stable at 2°C to 8°C
- Does not require freezer storage
- Ready for immediate use
Applications
- Phosphoproteomics and signaling pathway analysis
- Cell lysis and protein extraction
- Immunoprecipitation and kinase assays
- Western blotting and phosphorylation studies
- Tissue protein extraction workflows
Mechanism of Action
Protein phosphorylation is a critical regulatory mechanism in cellular processes such as signal transduction, cell division, and apoptosis. Upon cell lysis, endogenous phosphatases can rapidly dephosphorylate proteins, altering their function.
The Halt™ Phosphatase Inhibitor Cocktail contains a combination of inhibitors that target multiple phosphatase classes:
- Sodium fluoride – inhibits serine/threonine phosphatases
- Sodium orthovanadate – inhibits tyrosine phosphatases
- Sodium pyrophosphate – broad phosphatase inhibition
- β-glycerophosphate – inhibits serine/threonine phosphatases
This combination ensures comprehensive inhibition and preservation of phosphorylation states during experimental workflows.
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Type | Phosphatase Inhibitor Cocktail |
| Format | Liquid (100X concentrate) |
| Inhibitors | Phosphatase inhibitors |
| Quantity Options | Multiple pack sizes available |
| Working Concentration | 1X (adjustable to 2–3X if required) |
| Compatibility | BCA, Bradford assays, lysis buffers |
| Dialyzable | Yes |
| Storage | 2°C to 8°C (Do not freeze) |
| Product Line | Halt™ |
| Unit Size | Each |
Product Options
| Catalog Number | Description | Pack Size |
|---|---|---|
| 78420 | Single-use cocktail | 1 mL |
| 78428 | Single-use aliquots | 24 × 100 μL |
| 78426 | Multi-pack | 5 × 1 mL |
| 78427 | Bulk format | 10 mL |
Usage Instructions
- Add to lysis buffer at 1:100 dilution (1X final concentration)
- For high phosphatase activity, increase to 2X–3X concentration
- Mix thoroughly before use
- Proceed with protein extraction workflow
Important Notes
- Does not contain protease inhibitors
- For complete protection, combine with protease inhibitor cocktails
- Dialyze or desalt if incompatibility arises with downstream applications
Related Products
- Pierce™ Phosphatase Inhibitor Mini Tablets
- Halt™ Protease and Phosphatase Inhibitor Cocktail
- Thermo Scientific™ Pierce Cell Lysis Reagents
Thermo Scientific™ Halt™ Phosphatase Inhibitor Single-Use Cocktail provides a reliable and convenient solution for preserving protein phosphorylation during laboratory workflows. Its broad-spectrum inhibition, flexible concentration, and compatibility with downstream assays make it an essential reagent for phosphoproteomics and signaling research.
- Pack Size: 1 mL 10 mL 5 x 1 mL 24 x 100 μL