Thermo Scientific™ Pierce™ Protease and Phosphatase Inhibitor Mini Tablets, EDTA-free

Thermo Scientific™ Pierce™ Protease and Phosphatase Inhibitor Mini Tablets, EDTA-free, provide a complete and convenient solution for protecting proteins during cell lysis, extraction, and purification workflows. Each tablet contains a broad-spectrum combination of protease and phosphatase inhibitors, ensuring preservation of both protein integrity and phosphorylation status.

Optimized for precision applications, each mini tablet is sufficient for 10 mL of buffer or lysate, making it ideal for small-scale and sensitive experimental workflows where accuracy and control are critical.

Key Features

Dual Protection System

• Inhibits proteases and phosphatases simultaneously
• Prevents both protein degradation and dephosphorylation
• Maintains structural and functional integrity of proteins

Broad-Spectrum Protease Inhibition

• Targets serine, cysteine, and aspartic proteases
• Contains aprotinin, bestatin, E-64, and leupeptin
• Ensures comprehensive protection during extraction

Effective Phosphatase Inhibition

• Inhibits serine/threonine and tyrosine phosphatases
• Includes sodium fluoride, sodium orthovanadate, sodium pyrophosphate, and β-glycerophosphate
• Preserves phosphorylation-dependent signaling pathways

EDTA-Free Formulation

• Suitable for applications requiring divalent cations
• Compatible with metalloproteins and enzyme activity studies
• Reduces interference with metal-dependent assays

Convenient Mini Tablet Format

• Pre-measured tablets for precise dosing
• Dissolves quickly to form a clear solution
• One tablet treats 10 mL of buffer or lysate

Compatible with Downstream Applications

• Compatible with Pierce BCA Protein Assay
• Suitable for mass spectrometry workflows (no AEBSF)
• Can be used with most detergent-based lysis buffers

Applications

• Protein extraction and purification
• Cell lysis for signaling pathway studies
• Phosphoproteomics and kinase research
• Western blotting and immunoassays
• Mass spectrometry-based proteomics

Mechanism of Action

During cell lysis, proteases degrade proteins while phosphatases remove critical phosphate groups, altering protein function and signaling pathways. These mini tablets combine inhibitors that target both enzyme classes:

Protease inhibitors:

• Aprotinin – serine proteases
• Bestatin – aminopeptidases
• E-64 – cysteine proteases
• Leupeptin – serine and cysteine proteases

Phosphatase inhibitors:

• Sodium fluoride – serine/threonine phosphatases
• Sodium orthovanadate – tyrosine phosphatases
• Sodium pyrophosphate – broad phosphatase inhibition
• β-glycerophosphate – serine/threonine phosphatases

This dual-action system ensures comprehensive protection of proteins and their post-translational modifications.

Technical Specifications

Usage Instructions

• Add 1 tablet to 10 mL of lysis buffer or sample
• Allow to dissolve completely before use
• Use immediately in protein extraction or analysis workflows

Product Information

Storage and Stability

• Store at 2°C to 8°C
• Stable for up to 12 months under recommended conditions
• Avoid moisture exposure

Related Products

• Thermo Scientific Pierce Protease Inhibitor Mini Tablets EDTA-free
• Thermo Scientific Pierce Phosphatase Inhibitor Mini Tablets

Thermo Scientific™ Pierce™ Protease and Phosphatase Inhibitor Mini Tablets, EDTA-free, deliver a complete and efficient solution for protecting proteins and their phosphorylation states during laboratory workflows. With dual inhibition, high compatibility, and a convenient mini tablet format, they are ideal for advanced proteomics and signaling studies requiring precise and reliable results.