Thermo Scientific™ B-PER™ Bacterial Protein Extraction Reagent

The Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent is a ready-to-use, nonionic detergent-based solution designed for the efficient lysis of bacterial cells and extraction of native or recombinant proteins without denaturation. This versatile reagent simplifies protein extraction workflows, making it compatible with a wide range of downstream applications, including affinity chromatography, SDS-PAGE, and protein quantification assays.

Key Features:

1. All-Inclusive Formulation:
   • Contains lysozyme and a universal nuclease, enabling efficient cell lysis and DNA digestion for both Gram-positive and Gram-negative bacteria.
   • Reduces sample viscosity and increases protein yield, including large molecular weight (MW) proteins.
2. Mild, Non-Denaturing Extraction:
   • Maintains the native structure of proteins for compatibility with downstream applications.
   • Prepared in a Tris buffer with a proprietary nonionic detergent for gentle and effective cell disruption.
3. Ready-to-Use:
   • Convenient, one-step protocol for bacterial cell lysis.
   • Eliminates the need for traditional sonication or homemade lysis buffers.
4. High Compatibility:
   • Fully compatible with protease inhibitors, salts, reducing agents, denaturants, and chelating agents.
   • Yields lysates that work seamlessly with protein assays, affinity purification methods (e.g., GST, His-tag), immunoprecipitation, and protein interaction studies.
5. Improved Workflow Efficiency:
   • Provides quick and easy protein extraction with a simple 10–15 minute incubation.
   • Extracted proteins are directly suitable for downstream applications such as electrophoresis, crosslinking, and protein labeling.
6. Flexible and Scalable:
   • Suitable for any scale of protein extraction, from small research samples to larger production volumes.
   • Available in multiple buffer formulations (1X, 2X, phosphate-buffered options) to suit specific experimental needs.

Specifications:

Available Sizes:

Applications:

1. Protein Extraction:
   • Efficient recovery of native and recombinant proteins from bacterial lysates.
   • Suitable for purifying inclusion bodies to near-homogeneous levels.
2. Molecular Biology:
   • Facilitates cell lysis for genomic and proteomic studies.
   • Prepares lysates compatible with protein assays (e.g., Pierce BCA Protein Assay, Pierce 660nm Protein Assay).
3. Affinity Purification:
   • Ideal for downstream workflows, including immobilized metal affinity chromatography (IMAC) and glutathione chromatography (GST-tagged proteins).
4. Protein Analysis:
   • Produces lysates compatible with electrophoresis (SDS-PAGE), immunoprecipitation, and protein interaction studies.
5. Biotechnology Research:
   • A cost-effective alternative to similar reagents like Merck Millipore BugBuster™ Master Mix.

Protocol:

1. Preparation:
   • Harvest bacterial cells by centrifugation and remove the supernatant.
   • Resuspend the pellet in B-PER Complete Reagent.
2. Lysis:
   • Incubate the sample with mixing for 10–15 minutes at room temperature.
3. Protein Recovery:
   • Centrifuge to pellet cell debris and collect the supernatant containing soluble proteins.
4. Downstream Applications:
   • Use the lysate directly for affinity purification, protein assays, or other workflows.

Advantages:

1. Convenience:
   • Saves time by combining cell lysis, DNA digestion, and protein solubilization in a single step.
   • Eliminates the need for additional lysozyme or nuclease.
2. Enhanced Yields:
   • Efficiently extracts proteins, including large molecular weight targets.
3. Cost-Effective:
   • Provides a reliable alternative to more expensive reagents without compromising performance.
4. Compatibility:
   • Works with standard protein quantification assays and purification methods, ensuring smooth integration into existing workflows.

Comparison to Similar Products:

• Improved over homemade buffers: Avoids interfering substances and ensures consistent results.
• More effective than sonication: Reduces risks of protein denaturation and heat generation.
• Cost-Effective Equivalent: Comparable to Merck Millipore BugBuster™ Master Mix at a lower cost.

The Thermo Scientific™ B-PER™ Complete Bacterial Protein Extraction Reagent simplifies protein extraction workflows while maintaining compatibility with a broad range of downstream applications. Its ready-to-use, gentle formulation ensures high yields of native proteins, making it an indispensable tool for research laboratories and biomanufacturing facilities.