0
Biologix® TBE Buffer (1×), pH 8.3±0.1 – 500 mL
Catalog No :
CAS Number :
Brand :
In Stock
Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Biologix | ||
| Product Grade | Molecular Biology | ||
The Biologix® TBE Buffer (1×) is a high-quality, ready-to-use electrophoresis buffer formulated for reliable nucleic acid separation in agarose and polyacrylamide gel electrophoresis. Manufactured using purity-tested reagents and subjected to stringent quality control, this buffer ensures consistent performance and reproducibility across molecular biology workflows.
With a precisely controlled pH of 8.3 ±0.1, the buffer provides optimal ionic strength and buffering capacity, making it particularly suitable for high-resolution separation of small DNA and RNA fragments.
Key Features & Benefits
High Purity & Quality Assurance
- Filtered through 0.22 µm membranes for superior clarity and cleanliness
- DNase-free, RNase-free, protease-free, and pyrogen-free
- Ensures integrity of sensitive nucleic acid samples
Ready-to-Use Convenience
- Supplied as 1× working solution, eliminating dilution steps
- Reduces preparation time and minimizes risk of concentration errors
- Ideal for routine and high-throughput laboratory workflows
Optimized for Electrophoresis Performance
- Provides stable buffering capacity at pH 8.3
- Supports consistent migration patterns and band sharpness
- Suitable for both agarose and polyacrylamide gel electrophoresis
Enhanced Resolution for Small Fragments
- Particularly effective for resolving small molecular weight nucleic acids
- Ideal for applications requiring high-resolution DNA separation
Applications
- Agarose gel electrophoresis for DNA and RNA analysis
- Polyacrylamide gel electrophoresis (PAGE)
- PCR product analysis and verification
- DNA fragment separation and visualization
- Molecular biology and genomics workflows
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Type | TBE Buffer (1×) |
| Composition | Tris-Borate-EDTA |
| pH | 8.3 ±0.1 |
| Volume | 500 mL |
| Filtration | 0.22 µm filtered |
| Purity | DNase/RNase/Protease/Pyrogen-free |
| Application | Nucleic acid electrophoresis |
| Format | Ready-to-use solution |
| Storage Temperature | 2–30°C |
| Shelf Life | 36 months |
Performance Guidance (TBE vs TAE)
- TBE Buffer: Preferred for high-resolution separation of small DNA fragments and sharper band definition
- TAE Buffer: Recommended for large DNA fragments (>13 kb) and faster migration
Unique Advantages
- Ready-to-use format minimizes preparation errors
- High purity ensures protection of nucleic acid samples
- Consistent electrophoresis performance and reproducibility
- Suitable for both routine and advanced molecular biology applications
- Long shelf life for efficient laboratory inventory management
The Biologix® TBE Buffer (1×), pH 8.3 is a dependable and high-performance solution for laboratories requiring accurate, reproducible nucleic acid electrophoresis results. Its ready-to-use formulation, high purity, and optimized buffering capacity make it an essential reagent for DNA and RNA analysis workflows.