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Thermo Scientific™ TRIS-Buffered Saline (TBS, 10X) with 0.5% Tween 20
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Concentrated Wash Buffer for Western Blotting, ELISA, and Immunoassays
Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | Ambient | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | Formula | C₄H₁₁NO₃ |
Thermo Scientific™ TRIS-buffered saline (TBS, 10X) with 0.5% Tween 20 is a high-quality concentrated detergent buffer widely used in molecular biology and immunodetection workflows. This ready-to-use solution combines the buffering capacity of Tris-saline with the gentle nonionic surfactant Tween 20, helping to reduce nonspecific binding and improve washing efficiency in protein-based assays.
Supplied as a colourless viscous liquid, this 10X concentrate is ideal for laboratories seeking convenient preparation of working-strength TBS-T wash buffer for routine Western blotting and related applications.
Key Features and Benefits
Optimized for Immunodetection Protocols
TBS-T is commonly used as a wash buffer in Western blotting, ELISA, and other antibody-based detection methods to enhance signal clarity and reduce background.
Convenient 10X Concentrated Format
Delivered as a 10X stock solution, allowing easy dilution to 1X working concentration for daily laboratory use.
Contains Tween 20 for Improved Washing
The inclusion of 0.5% Tween 20 supports efficient removal of unbound antibodies while preserving antigen-antibody interactions.
High-Quality and Ready-to-Use
Supplied as a stable, colourless viscous liquid, eliminating the need for buffer weighing, mixing, or pH adjustment.
Physical Specifications
| Parameter | Description |
|---|---|
| Product Name | TRIS-buffered saline (TBS, 10X) with 0.5% Tween 20 |
| Physical Form | Viscous liquid |
| Appearance | Colourless |
| MDL Number | MFCD00678198 |
Applications
Thermo Scientific™ TBS-T (10X) is suitable for:
- Western blot washing steps
- Antibody dilution buffer preparation
- ELISA wash protocols
- Immunoassays requiring low background
- General protein blotting and detection workflows
- Laboratory buffer preparation convenience
Ordering Information
| Catalog Number | Description | Pack Size |
|---|---|---|
| J60448.AP | TRIS-buffered saline (TBS, 10X), with 0.5% Tween 20 | 500 mL |
| J60448.K2 | TRIS-buffered saline (TBS, 10X), with 0.5% Tween 20 | 1 L |
| J60448.K3 | TRIS-buffered saline (TBS, 10X), with 0.5% Tween 20 | 2 L |
Thermo Scientific™ TRIS-buffered saline (TBS, 10X) with 0.5% Tween 20 is a reliable and convenient concentrated wash buffer designed for high-performance Western blotting and immunoassay applications. With its ready-to-use viscous liquid format, consistent quality, and optimized detergent content, it provides laboratories with an efficient solution for reducing background and ensuring reproducible immunodetection results.
- Pack Size: 1L 500 mL 2L