Thermo Scientific™ TRIS-Buffered Saline (TBS, 20X) with 1% Tween 20

Thermo Scientific™ TRIS-buffered saline (TBS, 20X) with 1% Tween 20 is a high-quality concentrated wash buffer formulated for demanding immunodetection and protein analysis workflows. Commonly referred to as TBST, this solution combines the buffering strength of Tris-saline with the washing efficiency of the nonionic detergent Tween 20, providing excellent background reduction and consistent assay performance.

Supplied as a ready-to-use liquid concentrate, this TBST buffer is ideal for laboratories performing routine Western blotting, ELISA assays, and antibody-based detection protocols, where reproducibility and convenience are essential.

Key Features and Benefits

Optimized for Immunodetection Workflows

TBST is widely used as a wash and dilution buffer in Western blotting and ELISA protocols, helping to improve signal clarity by minimizing nonspecific antibody binding.

Contains 1% Tween 20 for Enhanced Washing

The inclusion of 1% Tween 20 increases detergent strength, improving removal of excess antibodies and unbound proteins while maintaining target integrity.

Convenient 20X Concentrated Format

Supplied as a 20X stock solution, allowing rapid dilution to 1X working strength and reducing preparation time in busy laboratories.

Stable Tris Buffer System

Tris maintains a consistent pH environment suitable for protein-based assays and immunological reactions.

Easy Storage and Handling

Designed for storage at ambient temperature, simplifying routine laboratory logistics.

Chemical Identity

The buffering component is based on Tris(hydroxymethyl)aminomethane:

Applications

Thermo Scientific™ TBST (20X) with Tween 20 is suitable for:

• Western blot membrane washing
• Antibody dilution buffer preparation
• ELISA microplate washing steps
• Immunoassay protocols requiring low background
• Protein blotting and detection workflows
• General laboratory detergent wash applications

Ordering Information

Thermo Scientific™ TRIS-buffered saline (TBS, 20X) with 1% Tween 20 is a reliable, concentrated TBST wash buffer designed for high-performance Western blotting and immunoassay applications. With enhanced detergent content, stable Tris buffering, and a convenient ready-to-dilute format, it supports reproducible results, reduced background noise, and efficient laboratory workflows.