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Thermo Scientific™ TRIS-Buffered Saline (TBS, 20X) with 2% Tween 20
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High-Strength TBST Wash Buffer for Immunoassays, Western Blotting, and Sample Preparation
Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | Ambient | ||
| Product Type | Buffer | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | Formula | C₄H₁₁NO₃ |
Thermo Scientific™ TRIS-buffered saline (TBS, 20X) with 2% Tween 20 is a concentrated, high-performance wash buffer designed for demanding immunoassay and protein detection workflows. Commonly referred to as TBST, this solution combines the buffering capacity of Tris-saline with an enhanced concentration of the nonionic detergent Tween 20, providing superior reduction of nonspecific binding and protein–protein interactions.
This buffer is widely used for washing steps in Western blotting, ELISA, and general immunodetection assays, as well as for sample preparation and dilution of secondary antibodies or streptavidin conjugates.
Supplied as a convenient ready-to-use 20X concentrate, it allows rapid preparation of working-strength wash buffer while ensuring reproducible results.
Key Features and Benefits
Enhanced Background Reduction with 2% Tween 20
The increased detergent content helps minimize nonspecific antibody binding and improves washing efficiency in sensitive immunoassays.
Ideal for Washing and Sample Preparation
Used extensively as a wash buffer between immunoreaction steps and for preparing antibody or conjugate incubation solutions.
Stable Tris-Saline Buffer System
TBS provides consistent ionic strength and pH stability, supporting optimal antigen–antibody interactions.
Convenient Concentrated Format
Delivered as a 20X stock solution, reducing preparation time and ensuring easy dilution to 1X working concentration.
Water Soluble and Easy to Handle
Fully soluble in water and suitable for routine laboratory workflows.
Research Use Only
Intended for laboratory research applications (RUO).
Applications
Thermo Scientific™ TBS (20X) with 2% Tween 20 is suitable for:
- Western blot membrane washing
- ELISA and immunoassay wash steps
- Reduction of nonspecific binding in antibody assays
- Dilution of secondary antibodies
- Preparation of streptavidin-AP conjugate solutions
- General immunodetection sample preparation workflows
Formulation Notes
- 1.0 M Tris-HCl
- 3.0 M NaCl
- pH 7.4 ± 0.1
- 2.0% Tween 20
Product Specifications
| Parameter | Description |
|---|---|
| Product Name | TRIS-buffered saline (TBS) with 2% Tween 20 |
| Concentration | 20X Stock Solution |
| Physical Form | Liquid |
| Solubility | Soluble in water |
| Recommended Storage | Ambient temperatures |
| Intended Use | Research Use Only (RUO) |
Ordering Information
| Catalog Number | Pack Size | Alternate Name |
|---|---|---|
| J60497.K2 | 1 L | J60497-K2 |
| J60497.K3 | 2 L | J60497-K3 |
Thermo Scientific™ TRIS-buffered saline (TBS, 20X) with 2% Tween 20 is a robust and highly effective TBST wash buffer for immunoassay workflows requiring enhanced detergent strength. By reducing nonspecific binding and improving washing efficiency, it supports clearer results and higher assay reproducibility in Western blotting, ELISA, and antibody-based detection protocols. Its convenient concentrated format makes it an essential reagent for modern research laboratories.
- Pack Size: 1L 2L