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Invitrogen™ PureLink™ HiPure Plasmid Filter Midiprep Kit
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Specifications:
| Application | DNA Extraction and Purification | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ HiPure Plasmid Filter Midiprep Kit is designed for rapid, high-yield purification of transfection-grade plasmid DNA from E. coli cultures ranging from 25 to 100 mL. Combining lysate clarification and anion-exchange DNA purification into a single step, the HiPure Filter Columns eliminate centrifugation, simplify workflows, and deliver DNA of purity equivalent to two CsCl gradient passes.
The protocol is safe and efficient, avoiding phenol, chloroform, ethidium bromide, and cesium chloride. With yields up to 200 μg of plasmid DNA per prep, the kit provides the quality and quantity required for the most demanding molecular biology applications.
Key Features
- Integrated Filter Columns – Combines lysate clarification and DNA binding in a single step.
- High Yields – Recover up to 200 μg plasmid DNA from midiprep cultures.
- Fast Workflow – Complete plasmid prep in ~90 minutes.
- Exceptional Purity – DNA quality equivalent to CsCl gradients, free from RNA, proteins, and endotoxins.
- Safe & Eco-Friendly – Eliminates hazardous solvents and reagents.
- Transfection-Grade DNA – Ultrapure plasmid DNA suitable for sensitive downstream applications.
Applications
- Mammalian cell transfections requiring high-purity, low-endotoxin DNA.
- Cloning and ligation workflows.
- Automated and manual sequencing.
- PCR amplification and in vitro transcription.
- Bacterial cell transformation.
- Restriction enzyme digestion and nucleic acid labeling.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Plasmid Filter Midiprep Kit |
| Catalog Numbers | K210014 (25 preps), K210015 (50 preps) |
| Column Type | HiPure Filter Columns (filtration + anion exchange) |
| Sample Volume | 25–100 mL overnight E. coli culture |
| Prep Scale | 100–200 μg plasmid DNA |
| Yield | Up to 200 μg (midiprep) |
| DNA Purity | Transfection-grade (CsCl gradient equivalent) |
| Elution Volume | 5 mL |
| Plasmid Types | High copy, low copy, BAC, <40 kb |
| Test Time | ~90 min |
| Isolation Technology | Anion-exchange resin with integrated filtration |
| Applications | Sequencing, cloning, transfection, PCR, in vitro transcription |
| Shipping Condition | Room temperature |
| Storage Condition | Room temperature |
| Unit Size | Each kit (25 or 50 preps) |
Kit Contents
- HiPure Filter Columns
- Equilibration Buffer (EQ1)
- Resuspension Buffer (R3)
- Lysis Buffer (L7)
- Precipitation Buffer (N3)
- RNase A
- Wash Buffer (W8)
- Elution Buffer (E4)
- TE Buffer
Unique Advantages
- Combines two workflow steps into one, saving time and minimizing sample handling.
- Provides endotoxin-free DNA suitable for transfection.
- Higher recovery and purity than standard silica miniprep systems.
- Available in convenient midiprep (25 or 50 prep) formats.
The Invitrogen™ PureLink™ HiPure Plasmid Filter Midiprep Kit streamlines plasmid DNA purification with its integrated filter column system, delivering transfection-grade DNA with high yields and exceptional purity. By eliminating centrifugation and hazardous chemicals, this kit provides a safer, faster, and more efficient alternative to traditional purification methods—ideal for sequencing, cloning, PCR, transfections, and other sensitive applications.
- Pack Size: For 50 Preps For 25 preps