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Invitrogen™ PureLink™ HiPure Plasmid Midiprep Kit
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Specifications:
| Application | Nucleic Acid Extraction | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ PureLink™ HiPure Plasmid Midiprep Kit enables efficient purification of transfection-grade plasmid DNA from E. coli cultures. Using anion-exchange resin technology, this kit produces DNA of exceptional purity—equivalent to two cesium chloride gradient passes—without the need for organic solvents, CsCl, or hazardous reagents.
Designed for medium-scale plasmid preps, the kit yields 100–350 μg of high-quality plasmid DNA from 15–25 mL bacterial cultures in less than 2 hours. The DNA exhibits A260/A280 > 1.8 and low endotoxin levels (0.1–1 EU/µg), making it ideal for mammalian transfection and other sensitive downstream applications.
Key Features
- High Purity – Produces transfection-grade DNA suitable for mammalian cells.
- Fast Processing – Complete plasmid purification in less than 2 hours.
- High Yield – Recovers 100–350 μg plasmid DNA from 15–25 mL overnight cultures.
- Safe & Efficient – Avoids hazardous chemicals such as phenol, chloroform, CsCl, and EtBr.
- Versatile – Suitable for a wide range of plasmid types and sizes, including BACs, bacmids, and ssM13 DNA.
- Trusted Technology – Proprietary anion-exchange resin ensures high recovery and efficient endotoxin removal.
Applications
- Mammalian cell transfections requiring endotoxin-free DNA.
- Cloning and ligation workflows.
- Automated and manual DNA sequencing.
- In vitro transcription and PCR amplification.
- Bacterial transformation.
- Restriction enzyme digestion and nucleic acid labeling.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Plasmid Midiprep Kit |
| Catalog Numbers | K210004 (25 preps), K210005 (50 preps) |
| Isolation Technology | Anion-exchange resin |
| Sample Type | E. coli bacterial culture |
| Sample Volume | 15–25 mL |
| Prep Scale | Medium-scale plasmid prep (100–200 μg DNA) |
| Yield | 100–350 μg plasmid DNA |
| DNA Quality | A260/A280 > 1.8 |
| Endotoxin Level | 0.1–1 EU/μg |
| Purity | Transfection grade |
| Elution Volume | 5 mL |
| Plasmid Types | High copy, low copy, BAC, bacmid, ssM13 DNA, <40 kb |
| Test Time | ~2 hours |
| High-Throughput | Manual use only |
| Shipping Condition | Room temperature |
| Storage | Room temperature |
| Unit Size | Each kit (25 or 50 preps) |
Kit Contents
- Resuspension Buffer (R3)
- RNase A
- Lysis Buffer (L7)
- Precipitation Buffer (N3)
- Equilibration Buffer (EQ1)
- Wash Buffer (W8)
- Elution Buffer (E4)
- TE Buffer
- HiPure Columns
- Column Holders
Unique Advantages
- Endotoxin-free plasmid DNA with high recovery rates.
- Safer and more efficient than cesium chloride or organic solvent methods.
- Ideal for both research-grade cloning and sensitive mammalian cell experiments.
- Consistently high purity across multiple plasmid types.
The Invitrogen™ PureLink™ HiPure Plasmid Midiprep Kit is a medium-scale solution for isolating transfection-grade plasmid DNA from E. coli cultures. Offering high yield, low endotoxin levels, and exceptional purity, it is ideal for demanding applications such as mammalian cell transfection, sequencing, cloning, and in vitro transcription. Its anion-exchange resin technology delivers consistent results while eliminating the hazards of traditional plasmid prep methods.
- Pack Size: For 50 Preps For 25 preps