Invitrogen™ Attune™ NxT Flow Cytometer, blue/red/violet/yellow (4 Lasers)

High-Speed Acoustic Focusing Flow Cytometry with Flexible, Scalable Multicolor Capabilities

The Invitrogen™ Attune™ NxT Flow Cytometer is a high-performance, modular flow cytometry system engineered to deliver speed, sensitivity, and flexibility for demanding multicolor applications. Featuring a cutting-edge acoustic focusing technology combined with hydrodynamic focusing, the Attune NxT enables rapid acquisition—up to 35,000 events per second—with minimal sample consumption and reduced clogging risks.

This configuration includes four lasers (488 nm Blue, 638 nm Red, 405 nm Violet, and 561 nm Yellow) and supports up to 14 fluorescence detection channels and 16 total parameters, offering a powerful platform for complex immunophenotyping, rare event detection, cell cycle analysis, and more.

Key Features and Benefits

• Acoustic Focusing Technology
  Replaces traditional sheath pressure-only systems with sound wave-based focusing, enabling faster, more stable sample alignment for consistent data and minimal sample loss.
• High-Speed Acquisition
  Analyze dilute or limited samples with acquisition speeds up to 35,000 events/sec (with <10% coincidence), and a maximum electronic speed of 65,000 events/sec.
• Modular & Upgradeable Design
  Start with the configuration you need now—expand later. The instrument supports field-upgradeable lasers, customizable filters, and autosampler integration.
• Small Footprint, Big Capability
  Designed for benchtop use or even inside a cell culture hood, the Attune NxT fits tight spaces without compromising throughput or performance.
• Enhanced Workflow Efficiency
  New CytKick™ and CytKick Max™ autosamplers accelerate high-throughput screening. Updated software is 21 CFR Part 11 compliant for regulated environments and runs on a Windows 10 platform.
• Clog-Resistant Sample Handling
  A syringe-driven system capable of 75 psi allows smooth acquisition of challenging samples like cardiomyocytes, CSF, or bone marrow without clogging or dilution steps.
• Flexible Sample Compatibility
  Supports traditional tubes, microplates (with autosampler), and varied sample types, from peripheral blood to rare stem cells, with or without washing or lysing.

Applications

The Attune NxT Flow Cytometer is optimized for a wide range of advanced research and translational applications, including:

• Multicolor Immunophenotyping
  Complex immune profiling in human and animal models with up to 14 fluorophores.
• Cell Cycle & Signaling Analysis
  Quantitative analysis of DNA content, apoptosis, and intracellular markers.
• Stem Cell Research
  Detection and quantification of rare progenitor and stem cell populations.
• Cancer & Apoptosis Studies
  Study of tumor heterogeneity, apoptotic markers, and therapeutic response.
• Microbiological Assays
  Enumeration, viability, and identification of bacteria, yeast, and fungi.
• Rare Event Detection
  Ideal for low-frequency events such as circulating tumor cells or minimal residual disease.
• No-Wash/No-Lyse Protocols
  Combine with No-Wash, No-Lyse Filter Kit for gentle and fast preparation of whole blood or other fragile samples.

Technical Specifications

Included Accessories and Reagents

• Attune™ NxT Flow Cytometer (Configured with 4 lasers)
• Minitower Windows 10 (64-bit) computer with preinstalled software
• Power cord with adapters
• USB cable
• Attune™ Shutdown Solution
• Attune™ Wash Solution
• Attune™ Focusing Fluid
• Attune™ NxT Flow Cell Cleaning Solution
• Attune™ Performance Tracking Beads
• Quick Reference Card

Ordering Information

The Invitrogen™ Attune™ NxT Flow Cytometer (blue/red/violet/yellow configuration) offers a high-speed, highly adaptable solution for advanced flow cytometry applications. By combining acoustic focusing technology with field-upgradeable modularity, it enables researchers to push the limits of multicolor analysis with unmatched precision, speed, and flexibility. Its compact design, 21 CFR Part 11–ready software, and broad sample compatibility make it equally suitable for high-throughput core facilities, regulated labs, and focused research environments.