Invitrogen™ Attune™ NxT No-Wash No-Lyse Filter Kit

Streamlined Whole Blood Immunophenotyping Without Lysis or Wash Steps

The Invitrogen™ Attune™ NxT No-Wash No-Lyse Filter Kit is a specialized accessory for the Attune™ NxT Acoustic Focusing Cytometer, enabling rapid, gentle, and efficient analysis of whole blood samples without the need for red blood cell (RBC) lysis or centrifugation-based wash steps. This kit reconfigures the Attune NxT system to detect violet side scatter (VSSC)—unlocking a differential light scatter method ideal for identifying leukocyte populations in minimally processed samples.

By eliminating traditional sample preparation procedures, this kit enhances workflow speed, preserves cell integrity, and reduces sample loss, making it ideal for high-throughput immune profiling in translational and clinical research.

Key Features and Benefits

• Enables No-Wash, No-Lyse Whole Blood Analysis
  Facilitates immunophenotyping of whole blood without RBC lysis or washing, avoiding sample damage and cell loss typically associated with conventional preparation steps.
• Violet Side Scatter Configuration
  Adds an additional side scatter detection channel off the 405 nm violet laser, supporting differentiation of leukocytes from erythrocytes based on light scatter properties.
• Fast Data Acquisition from Dilute Samples
  Works in tandem with the Attune NxT’s acoustic focusing to allow high-speed data acquisition even at low cell concentrations.
• Simple Instrument Reconfiguration
  The kit enables quick installation of the included 415 LP dichroic mirror and 405/10 bandpass emission filter, allowing seamless switch to violet scatter mode when needed.
• Alternative Trigger Strategy
  Compatible with fluorescence-based or scatter-based triggering to exclude RBCs without requiring physical removal—saving time and reagents.

Application Highlights

• Whole Blood Immunophenotyping
  Identify and characterize lymphocytes, monocytes, neutrophils, and other cell populations directly from minimally processed blood.
• Preservation of Fragile Cell Populations
  Ideal for rare or sensitive immune cell subsets that may be lost or altered during centrifugation or lysis.
• High-Throughput Immune Monitoring
  Enables fast, standardized acquisition suitable for longitudinal or large-cohort studies.
• Translational Research
  Reduces variability and hands-on time in sample preparation, making it compatible with biobanking or regulated lab environments.

Technical Specifications

Kit Contents

• 1 × 415 nm Long Pass Dichroic Filter
• 1 × 405/10 nm Bandpass Emission Filter

The Attune™ NxT No-Wash No-Lyse Filter Kit transforms the Attune NxT Flow Cytometer into a powerful tool for high-throughput whole blood immunophenotyping without the drawbacks of traditional sample preparation. Leveraging violet side scatter and acoustic focusing, this kit simplifies workflows, minimizes cell loss, and enables rapid data acquisition from challenging or limited samples. It is an essential enhancement for laboratories seeking to streamline sample processing while maintaining analytical rigor.