Invitrogen™ Collibri™ Stranded RNA Library Cleanup Kit for Illumina™ Systems

The Invitrogen™ Collibri™ Library Cleanup Kit for Illumina™ Systems is a high-efficiency magnetic bead-based purification solution designed to clean and purify next-generation sequencing (NGS) libraries during library preparation workflows. Optimized for use with Illumina sequencing platforms, this kit ensures efficient removal of unwanted reaction components such as excess primers, adapter dimers, enzymes, nucleotides, and buffer contaminants while preserving high-quality sequencing-ready DNA or cDNA libraries.

Using advanced magnetic bead purification technology, the Collibri Library Cleanup Kit provides consistent recovery, excellent reproducibility, and high library integrity across a wide range of sequencing applications. This purification step is essential for improving sequencing efficiency, reducing background noise, and ensuring accurate downstream sequencing results.

The kit integrates seamlessly with the Collibri library preparation workflow and is suitable for RNA sequencing, transcriptome analysis, gene expression profiling, and other Illumina-compatible sequencing applications. It supports both manual and automated workflows, making it ideal for research laboratories, sequencing core facilities, and high-throughput genomics environments.

Key Features

• Efficient library purification: Removes primers, adapter dimers, enzymes, and contaminants
• Magnetic bead-based technology: Ensures high recovery and reproducibility of sequencing libraries
• Illumina-compatible workflow: Fully optimized for Illumina sequencing platforms including MiSeq™, NextSeq™, HiSeq™, and NovaSeq™
• Improves sequencing performance: Enhances library quality and sequencing accuracy
• Automation compatible: Suitable for manual and automated high-throughput workflows
• Supports multiplex library preparation: Compatible with indexed and multiplexed libraries
• Preserves library integrity: Maintains library complexity and sequencing readiness

Applications

• Next-generation sequencing (NGS) library preparation
• RNA sequencing (RNA-Seq) workflows
• Whole transcriptome sequencing
• Gene expression analysis
• cDNA library purification
• Library preparation for Illumina sequencing
• Multiplex sequencing workflows

Technical Specifications

Workflow Integration

Typical Illumina NGS workflow using Collibri reagents:

RNA Extraction → RNA Quantification → RNA Integrity Assessment → rRNA Depletion → Library Preparation → Library Cleanup → Library Quantification → Sequencing

Advantages

• Improves sequencing efficiency and data quality
• Ensures removal of adapter dimers and contaminants
• Provides consistent library recovery
• Reduces sequencing artifacts and background noise
• Essential step for reliable NGS library preparation

The Invitrogen™ Collibri™ Library Cleanup Kit is an essential component of Illumina-compatible NGS workflows, providing reliable and efficient purification of sequencing libraries. Its optimized magnetic bead-based purification ensures high recovery, improved library quality, and accurate sequencing results, making it ideal for transcriptomics, genomics, and molecular biology research laboratories performing high-quality next-generation sequencing.