Invitrogen™ Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems

The Invitrogen™ Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems is a comprehensive, high-performance solution designed for the preparation of strand-specific cDNA libraries for next-generation sequencing (NGS) on Illumina platforms. This kit enables rapid and reliable conversion of total RNA, mRNA-enriched RNA, or ribodepleted RNA into sequencing-ready libraries, supporting both whole transcriptome sequencing and targeted gene expression analysis across all species.

Leveraging advanced enzymatic technologies—including SuperScript™ IV Reverse Transcriptase, Dynabeads™ magnetic bead purification, and Platinum™ SuperFi™ DNA Polymerase—the Collibri kit delivers superior strand specificity (>98%), high sensitivity, and exceptional library quality. The optimized workflow enables preparation of sequencing-ready libraries in approximately 4.5 hours, improving efficiency in transcriptomics workflows while maintaining reproducibility and high data integrity.

The kit is available in multiple configurations, including options with integrated cleanup modules, rRNA depletion modules for human, mouse, and rat samples, and unique dual indexes (UDI) for multiplex sequencing. These flexible configurations allow laboratories to tailor workflows to their specific experimental needs, ranging from basic mRNA sequencing to full transcriptome profiling.

Key Features

• Complete RNA library preparation solution: Includes library prep, cleanup, and optional rRNA depletion and indexing modules
• High strand specificity (>98%): Enables accurate detection of coding and non-coding RNA transcripts
• Fast workflow: Generate sequencing-ready libraries in approximately 4.5 hours
• Broad RNA compatibility: Supports total RNA, mRNA-enriched RNA, ribodepleted RNA, and FFPE-derived RNA samples
• Illumina platform compatibility: Optimized for MiSeq™, NextSeq™, HiSeq™, and NovaSeq™ systems
• Multiplex sequencing support: Enables multiplexing of up to 96 samples using single or unique dual indexes
• Magnetic bead-based cleanup: Ensures high library recovery and purity
• Automation compatible: Suitable for high-throughput sequencing workflows
• Visual workflow indicators: Built-in dyes provide visual feedback during library preparation

Available Kit Configurations

Applications

• Whole transcriptome sequencing
• mRNA sequencing
• Gene expression profiling
• Differential gene expression analysis
• Alternative splicing analysis
• Transcript isoform detection
• Non-coding RNA analysis
• Cancer transcriptomics
• Biomarker discovery
• FFPE RNA sequencing

Technical Specifications

Workflow Integration

RNA Extraction → RNA Quantification → RNA Integrity Assessment → (Optional rRNA Depletion) → Library Preparation → Cleanup → Indexing → Library Quantification → Illumina Sequencing

Advantages Over Conventional RNA Library Prep Kits

• Faster workflow with fewer steps
• Higher strand specificity for accurate transcript analysis
• Improved detection of low-abundance transcripts
• Reduced sequencing artifacts
• Flexible configurations for different experimental needs
• Fully optimized for Illumina sequencing platforms

The Invitrogen™ Collibri™ Stranded RNA Library Prep Kit provides a complete, efficient, and reliable solution for generating high-quality RNA sequencing libraries for Illumina NGS platforms. With exceptional strand specificity, fast workflow, and flexible configurations including cleanup, indexing, and ribodepletion modules, this kit is ideal for transcriptomics, gene expression analysis, and advanced RNA sequencing applications in research, clinical, and genomics laboratories.