Thermo Scientific™ Invitrogen™ Collibri™ Stranded RNA Library Prep Kit with Indexes for Illumina™ Systems

The Invitrogen™ Collibri™ Stranded RNA Library Prep Indexes for Illumina™ Systems are high-quality indexing primers designed to enable multiplexing and accurate identification of RNA sequencing libraries in Illumina next-generation sequencing (NGS) workflows. These indexes allow multiple RNA samples to be uniquely barcoded and sequenced simultaneously within a single sequencing run, maximizing throughput while reducing sequencing costs.

Optimized for seamless integration with the Collibri Stranded RNA Library Preparation workflow, these indexing kits include both single-index and unique dual-index (UDI) configurations, providing flexibility to support a wide range of sequencing applications. Unique dual indexes significantly reduce index hopping and sample misassignment, ensuring greater data accuracy—particularly critical for high-throughput sequencing platforms such as Illumina NovaSeq™, NextSeq™, MiSeq™, and HiSeq™ systems.

The indexes are introduced during the PCR amplification step of library preparation using high-fidelity polymerase chemistry. This process incorporates Illumina-compatible adapter sequences and unique barcode combinations, allowing precise sample tracking and reliable multiplex sequencing. These indexing kits support multiplexing of up to 96 samples, making them ideal for transcriptomics, gene expression profiling, and large-scale RNA sequencing studies.

Designed for reliability and performance, the Collibri RNA Library Prep Indexes maintain high library integrity, enable efficient multiplexing, and ensure compatibility with Illumina sequencing workflows across a wide range of RNA sample types, including low-input and FFPE-derived RNA.

Key Features

• Supports multiplex RNA sequencing: Enables simultaneous sequencing of multiple samples in a single Illumina run
• Single-index and unique dual-index options: Provides flexibility and enhanced accuracy for different sequencing applications
• Reduces index hopping: Unique dual indexes improve sample identification accuracy on high-throughput sequencers
• Illumina-compatible indexing chemistry: Fully compatible with Illumina sequencing platforms
• Optimized for Collibri workflows: Integrates seamlessly with Collibri RNA Library Prep and Cleanup Kits
• High-fidelity indexing: Ensures accurate sample identification and reproducible results
• Supports high-throughput sequencing: Multiplex up to 96 RNA libraries
• Suitable for degraded and FFPE RNA samples

Applications

• RNA sequencing (RNA-Seq)
• Whole transcriptome sequencing
• Gene expression profiling
• Transcript isoform analysis
• Cancer transcriptomics
• Non-coding RNA sequencing
• Biomarker discovery
• Multiplex RNA sequencing workflows

Technical Specifications

Available Configurations

Workflow Integration

RNA Extraction → RNA Quantification → RNA Integrity Assessment → rRNA Depletion → Library Preparation → Cleanup → Indexing → Library Quantification → Illumina Sequencing

Advantages of Unique Dual Indexing

• Minimizes index hopping and sample misassignment
• Improves sequencing accuracy
• Enables high-confidence multiplex sequencing
• Essential for high-throughput Illumina platforms

The Invitrogen™ Collibri™ Stranded RNA Library Prep Indexes provide a reliable and efficient solution for multiplex RNA sequencing on Illumina platforms. With flexible single and unique dual indexing options, these kits enable accurate sample tracking, high-throughput sequencing, and improved data quality, making them an essential component of modern RNA-Seq workflows in genomics and transcriptomics research laboratories.