Invitrogen™ Collibri™ rRNA Depletion Kit for Illumina™ Systems

The Invitrogen™ Collibri™ rRNA Depletion Kit for Illumina™ Systems is a highly efficient ribosomal RNA (rRNA) removal solution designed to improve RNA sequencing performance by selectively depleting abundant rRNA molecules from total RNA samples. Optimized for use with Illumina next-generation sequencing (NGS) workflows, this kit enhances sequencing efficiency by enriching for informative RNA species such as messenger RNA (mRNA), long non-coding RNA (lncRNA), and other regulatory RNA transcripts.

Ribosomal RNA typically accounts for over 80–90% of total RNA in cells, which can significantly reduce sequencing depth for biologically relevant transcripts. The Collibri rRNA Depletion Kit addresses this challenge by selectively removing rRNA prior to library preparation, thereby increasing sequencing coverage, improving transcript detection sensitivity, and reducing overall sequencing costs.

This kit integrates seamlessly with the Collibri Stranded RNA Library Preparation workflow and is compatible with Illumina sequencing platforms including MiSeq™, NextSeq™, HiSeq™, and NovaSeq™. Its optimized chemistry ensures efficient depletion across a wide range of RNA sample types, including challenging or partially degraded samples such as FFPE-derived RNA.

Key Features

• Efficient ribosomal RNA removal: Selectively depletes abundant rRNA to improve transcriptome coverage
• Enhanced sequencing efficiency: Maximizes sequencing reads dedicated to informative RNA species
• Illumina-compatible workflow: Fully optimized for Illumina next-generation sequencing systems
• Supports strand-specific RNA sequencing: Maintains transcript orientation for accurate transcriptome analysis
• Broad RNA compatibility: Suitable for total RNA, ribodepleted RNA, and partially degraded RNA samples
• Optimized for Collibri workflows: Integrates seamlessly with Collibri RNA Library Prep and Cleanup Kits
• Improved sensitivity and data quality: Enhances detection of low-abundance transcripts
• Automation compatible: Suitable for both manual and automated high-throughput workflows

Applications

• Whole transcriptome RNA sequencing (RNA-Seq)
• Gene expression profiling
• Non-coding RNA analysis
• Transcript isoform identification
• Alternative splicing analysis
• Cancer transcriptomics research
• FFPE RNA sequencing workflows
• Biomarker discovery
• Functional genomics studies

Technical Specifications

Workflow Integration

Typical Illumina RNA sequencing workflow using Collibri kits:

RNA Extraction → RNA Quantification → RNA Integrity Assessment → rRNA Depletion → Library Preparation → Library Cleanup → Library Quantification → Sequencing

Advantages of rRNA Depletion

• Improves sequencing efficiency and data quality
• Increases sensitivity for low-abundance transcripts
• Reduces sequencing cost per informative read
• Enables comprehensive whole transcriptome analysis
• Essential for total RNA sequencing workflows

The Invitrogen™ Collibri™ rRNA Depletion Kit is an essential solution for preparing high-quality RNA samples for Illumina sequencing. By efficiently removing ribosomal RNA, the kit enhances transcriptome coverage, improves sequencing efficiency, and enables accurate detection of coding and non-coding RNA species. It is ideal for genomics, transcriptomics, and molecular biology laboratories performing advanced RNA sequencing applications.