Invitrogen™ Collibri™ Library Amplification Master Mix with Primer Mix

The Invitrogen™ Collibri™ Library Amplification Master Mix with Primer Mix is a high-fidelity, ready-to-use PCR solution designed for efficient and accurate amplification of next-generation sequencing (NGS) libraries for Illumina™ platforms. This optimized amplification system combines the advanced Platinum™ SuperFi™ DNA Polymerase, a proprietary amplification buffer, and a dedicated 10X Primer Mix targeting the P5 and P7 adapter regions, enabling seamless enrichment of adapter-ligated sequencing libraries.

This complete amplification system ensures uniform library amplification with minimal bias, high yield, and superior sequence accuracy. The proofreading activity of Platinum SuperFi DNA Polymerase delivers extremely low error rates—comparable to PCR-free workflows—ensuring reliable and reproducible sequencing results.

The included primer mix simplifies workflow setup by eliminating the need to prepare or source separate primers. Additionally, the master mix contains an inert blue dye and the primer mix contains a yellow dye; when combined, the reaction turns green, providing a visual confirmation of correct reaction assembly and reducing pipetting errors.

This master mix is ideal for RNA-Seq, DNA sequencing, and other Illumina NGS library preparation workflows, particularly when working with low-input libraries requiring highly accurate amplification.

Key Features

• Complete amplification solution: Includes master mix and primer mix targeting Illumina P5 and P7 adapter sequences
• Ultra-high fidelity amplification: Platinum SuperFi DNA Polymerase provides ≥100× higher fidelity than Taq polymerase
• Minimal amplification bias: Ensures uniform amplification across GC-rich and complex sequences
• High amplification efficiency: Generates high yields even from low-input libraries
• Hot-start PCR technology: Improves specificity and prevents nonspecific amplification
• Visual workflow confirmation: Blue and yellow dyes combine to produce green reaction mix for easy pipetting verification
• Optimized for Illumina sequencing workflows: Fully compatible with Illumina NGS library preparation
• Automation compatible: Suitable for manual and high-throughput workflows

Applications

• Next-generation sequencing (NGS) library amplification
• RNA sequencing (RNA-Seq) library preparation
• DNA sequencing library amplification
• cDNA library enrichment
• Illumina sequencing workflows
• Transcriptomics and genomics research
• Multiplex sequencing library preparation

Technical Specifications

Workflow Integration

Library Preparation → Cleanup → Library Amplification (Master Mix with Primer Mix) → Library Quantification → Illumina Sequencing

Advantages Over Standard PCR Master Mixes

• Designed specifically for NGS library amplification
• Includes optimized primers for Illumina adapter regions
• Superior amplification accuracy and efficiency
• Reduces preparation time and workflow complexity
• Minimizes amplification errors and sequencing artifacts

The Invitrogen™ Collibri™ Library Amplification Master Mix with Primer Mix provides a complete, high-performance solution for accurate and efficient amplification of Illumina-compatible sequencing libraries. Its high-fidelity polymerase, integrated primer mix, and optimized buffer system ensure reliable amplification, reduced workflow complexity, and superior sequencing results, making it an essential component of RNA-Seq and NGS library preparation workflows.