Invitrogen™ Collibri™ Library Quantification Kit

The Invitrogen™ Collibri™ Library Quantification Kit is a highly accurate, ready-to-use qPCR-based solution designed for precise quantification of next-generation sequencing (NGS) libraries prepared for Illumina™ sequencing platforms. This kit enables reliable measurement of amplifiable library molecules, ensuring optimal library loading concentrations and maximizing sequencing efficiency and data quality.

The kit includes a Collibri Library Quantification Master Mix, powered by Platinum™ II Taq Hot Start DNA Polymerase, along with a ready-to-use library dilution buffer. The master mix contains all essential qPCR components—including adapter-specific primers, SYBR™ Green detection dye, passive reference dye, dNTPs, and optimized reaction buffer—eliminating the need for reagent preparation and simplifying workflow setup.

Designed for universal compatibility, the kit features a standardized passive reference dye concentration, allowing seamless use with all major real-time PCR instruments such as Applied Biosystems™ QuantStudio™, 7500 Fast, 7900HT, StepOne™, StepOnePlus™, and ViiA™ 7 systems. This ensures accurate and reproducible quantification across different qPCR platforms.

The included dilution buffer stabilizes sequencing libraries during dilution, preserving library integrity. Additionally, built-in visual tracking dyes—blue in the master mix and yellow in the dilution buffer—combine to produce a green reaction mixture, allowing easy visual confirmation of proper pipetting and reducing the risk of setup errors.

Key Features

• Accurate qPCR-based library quantification: Ensures precise measurement of sequencing-ready libraries
• Ready-to-use master mix: Includes polymerase, primers, dyes, and buffer—no reagent preparation required
• Powered by Platinum II Taq Hot Start DNA Polymerase: Provides high specificity, sensitivity, and reproducibility
• Universal instrument compatibility: Works with all major real-time PCR systems without adjustment
• Adapter-specific primers included: Enables accurate quantification of Illumina-compatible libraries
• Visual workflow indicators: Colored dyes help confirm correct pipetting and reaction setup
• Library stabilization buffer included: Preserves library integrity during dilution
• Optimized for Illumina sequencing workflows

Applications

• Illumina NGS library quantification
• RNA sequencing (RNA-Seq) library quantification
• DNA sequencing library quantification
• Transcriptome sequencing workflows
• Next-generation sequencing library preparation workflows
• qPCR-based sequencing library quality control

Technical Specifications

Workflow Integration

Library Preparation → Cleanup → Amplification → Library Quantification (Collibri Quantification Kit) → Library Normalization → Illumina Sequencing

Advantages Over Spectrophotometric Quantification

• Measures only amplifiable library molecules
• Provides higher accuracy than NanoDrop or fluorometric methods alone
• Improves sequencing cluster density optimization
• Reduces sequencing failures and improves data quality

The Invitrogen™ Collibri™ Library Quantification Kit provides a precise, reliable, and easy-to-use solution for quantifying Illumina sequencing libraries using qPCR. Its optimized master mix, adapter-specific primers, and universal instrument compatibility ensure accurate quantification, enabling optimal sequencing performance and high-quality NGS data in genomics and transcriptomics workflows.