VIASURE® Dengue Virus Real Time PCR Detection Kit

The VIASURE Dengue Virus Real Time PCR Detection Kit is a highly sensitive and specific molecular assay designed for the qualitative detection of Dengue virus (DENV) RNA in clinical samples. Based on advanced real-time RT-PCR technology with fluorescent probe detection, the kit enables rapid and reliable identification of dengue infections, particularly during the early phase of the disease when viral RNA is most detectable.

Optimized for clinical and research laboratories, this assay supports accurate diagnosis when used alongside clinical symptoms and epidemiological data. Its room-temperature stability and flexible format configurations make it a practical and efficient solution for laboratories operating in both resource-rich and resource-limited environments.

Key Features

• High Sensitivity and Specificity
  Real-time RT-PCR assay ensures accurate detection of Dengue virus RNA, even at low viral loads.
• Fluorescent Probe-Based Detection
  Provides reliable and specific signal detection, minimizing false positives.
• Optimized for Early Infection Detection
  Ideal for identifying dengue during the acute phase (first 4–5 days of infection).
• Room Temperature Storage & Transport
  Eliminates cold-chain requirements, simplifying logistics and storage.
• Flexible Format Options
  Available in strip and plate formats to suit varying laboratory throughput and instrument compatibility.
• Streamlined Workflow
  Ready-to-use components reduce preparation time and minimize contamination risk.

Applications

Clinical Diagnostics (Research Use / Screening Support)

• Detection of Dengue virus in symptomatic patients
• Support for diagnosis of febrile illness
• Early-stage infection detection

Public Health & Surveillance

• Monitoring dengue outbreaks
• Epidemiological surveillance in endemic regions
• Screening of travelers from tropical regions

Molecular Research

• Arbovirus research and detection
• Viral load and infection studies
• Comparative studies with other flaviviruses (e.g., Zika)

Assay Principle

1. RNA Extraction from clinical specimens (serum, plasma, or blood cells)
2. Reverse Transcription and Amplification (RT-PCR)
3. Detection via Fluorescent Probes specific for Dengue virus
4. Real-Time Analysis for qualitative result interpretation

Technical Specifications

Ordering Information

Clinical & Scientific Background

Dengue virus (DENV), a member of the Flavivirus genus, is a mosquito-borne pathogen transmitted primarily by Aedes species. It represents a major global health concern, with millions of infections annually, particularly in tropical and subtropical regions.

Clinical presentation typically includes:

• High fever (2–7 days)
• Headache and retro-orbital pain
• Muscle and joint pain
• Skin rash
• Mild bleeding manifestations

The disease progresses through three phases:

1. Febrile Phase
2. Critical Phase (risk of plasma leakage and complications)
3. Convalescent Phase

During the early phase of infection, viral RNA is detectable in blood, making RT-PCR the preferred method for early diagnosis. Serological methods may be used later but can be limited by cross-reactivity with other flaviviruses such as Zika virus.

Unique Advantages

• Early and Reliable Detection
  Enables diagnosis during the acute phase when intervention is most critical.
• High Diagnostic Accuracy
  Minimizes cross-reactivity issues associated with serological tests.
• Operational Flexibility
  Multiple formats support diverse laboratory workflows and instrument platforms.
• Logistics-Friendly Design
  Room temperature stability is ideal for regions with limited cold-chain infrastructure.
• Supports Public Health Response
  Critical tool for outbreak monitoring and disease control programs.

The VIASURE Dengue Virus Real Time PCR Detection Kit is a robust and reliable molecular diagnostic solution for the detection of dengue infections. With its high sensitivity, early detection capability, and flexible formats, it is well-suited for clinical laboratories, research institutions, and public health programs—particularly in dengue-endemic regions.