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Thermo Scientific™ Triton™ X-100 Surfact-Amps™ Detergent Solution
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Specifications:
| Application | molecular biology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | ||
High-Purity, Stabilized Nonionic Detergent Solution for Protein Extraction and Cell Lysis Applications
Thermo Scientific™ Triton™ X-100 Surfact-Amps™ Detergent Solution is a pre-made 10% (w/v) aqueous solution of highly purified Triton™ X-100, a nonionic polyoxyethylene surfactant widely used in biochemical and molecular biology workflows. This ready-to-use solution is designed for maximum convenience, consistency, and purity, eliminating concerns of oxidation and degradation associated with bulk detergents.
Each batch is packaged under inert nitrogen in low-leach HDPE bottles or sealed glass ampules, maintaining stability and minimizing peroxides or carbonyl contaminants. It is ideal for researchers performing cell lysis, membrane solubilization, or protein extraction under non-denaturing conditions.
Key Features & Benefits
- High-Purity Triton™ X-100 – Precisely formulated and stabilized to ensure maximum reagent quality
- Ready-to-Use 10% Solution – Reduces weighing and dilution errors; saves time and effort
- Oxidation-Free Packaging – Stored under nitrogen to prevent peroxide and carbonyl formation
- Nonionic & Non-Denaturing – Solubilizes proteins while preserving function and structure
- Versatile Format Options – Available in glass ampules (10 mL) or HDPE bottles (50 mL, 250 mL, 1 L)
Applications
- Protein Extraction – Gentle solubilization of membrane and cytoplasmic proteins
- Cell and Organelle Lysis – Common component in non-denaturing lysis buffers
- Membrane Permeabilization – Enables controlled access to intracellular targets
- Immunoassays and ELISA – Used to block or wash membranes and plates
- Tissue Solubilization – Aids in sample preparation for Western blotting and proteomics
Technical Specifications
| Attribute | Specification |
|---|---|
| Product Name | Triton™ X-100 Surfact-Amps™ Detergent Solution |
| Catalog Numbers | 85111 (50 mL), 28314 (6 x 10 mL), 85112 (250 mL), 28313 (1 L) |
| Concentration | 10.0 ± 1.0% (w/v) |
| Form | Liquid (aqueous solution) |
| Detergent Type | Nonionic surfactant |
| Molecular Weight | 647 g/mol |
| CMC | 0.24 mM (0.0155% w/v) |
| Aggregation Number | ~140 |
| Micelle Molecular Weight | ~90,000 g/mol |
| Cloud Point | 64°C |
| Dialyzable | No |
| Oxidant Content | ≤1.0 μeq/mL peroxides |
| Carbonyl Content | ≤1.0 μeq/mL |
| Packaging | Nitrogen-sealed HDPE or ampules |
| Storage Conditions | 20°C to 25°C; protect from light |
| Regulatory Use | For Research Use Only |
Storage & Handling
- Store tightly sealed in the original container at 20–25°C
- Protect from direct sunlight and avoid prolonged exposure to air
- Shake gently before use if solution appears hazy
- Use appropriate PPE during handling; avoid skin and eye contact
Thermo Scientific™ Triton™ X-100 Surfact-Amps™ Detergent Solution provides a high-purity, stable, and convenient source of Triton X-100 for protein chemistry, immunoassays, and cell biology. Its nitrogen-sealed packaging and ultrapure composition make it a superior choice for applications requiring low background noise, gentle lysis, and reproducible solubilization of membrane proteins.
- Pack Size: 1L 50 mL 250 mL 6 x 10 mL