Abcam CHAPS Detergent, 10% Solution, Sterile-Filtered, >= 98%

High-Purity, Ready-to-Use Zwitterionic Detergent Solution for Membrane Protein Solubilization

Abcam™ CHAPS Detergent (10% Solution, Sterile-Filtered) is a high-purity, sterile-filtered aqueous solution of CHAPS (3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate), a non-denaturing zwitterionic detergent commonly used in membrane protein extraction, receptor solubilization, and electrophoresis applications. With a purity level of ≥98%, this reagent offers consistent performance in sensitive biochemical and proteomic workflows.

CHAPS combines the favorable solubilizing properties of bile salts and sulfobetaines, making it particularly effective in disrupting protein-protein interactions while preserving native protein structure.

Key Features & Benefits

• 10% (w/v) Pre-Made Solution – Ready-to-use, sterile-filtered format minimizes preparation time and contamination risk
• Zwitterionic & Non-Denaturing – Ideal for applications requiring preserved protein activity and structure
• High Purity (≥98%) – Suitable for sensitive and high-resolution analytical applications
• Broad Compatibility – Effective in cell lysis buffers, membrane extraction protocols, and isoelectric focusing (IEF)
• Synthetic Source – Consistent quality across batches for reproducible results

Applications

• Membrane Protein Solubilization – Efficiently extracts hydrophobic and membrane-associated proteins
• Protein-Protein Interaction Disruption – Disaggregates protein complexes under mild conditions
• Receptor Biochemistry – Solubilizes receptors while maintaining ligand-binding capability
• Isoelectric Focusing (IEF) – Frequently used in 2D electrophoresis for non-denaturing separation
• General Biochemistry – Cell lysis, protein extraction, and proteomic workflows requiring detergent compatibility

Technical Specifications

Abcam™ CHAPS Detergent, 10% Sterile-Filtered Solution provides a high-quality, convenient format for researchers working in membrane protein isolation, protein structure-function studies, and non-denaturing electrophoretic workflows. With ≥98% purity and sterile filtration, it ensures reliability and safety in high-sensitivity applications across biochemistry, molecular biology, and proteomics.