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Sigma-Aldrich™ CHAPS, Molecular Biology Grade
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Specifications:
| Application | molecular biology | ||
| Storage Temperature | -20°C | ||
| Product Type | Molecular Biology Reagent | Forms | Powder |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Molecular Biology | Formula | C₃₂H₅₈N₂O₇S |
High-Purity Zwitterionic Detergent for Membrane Protein Solubilization, Isoelectric Focusing, and 2D Electrophoresis
Sigma-Aldrich™ CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) is a high-purity, non-denaturing zwitterionic detergent widely used in membrane protein biochemistry and electrophoretic applications. Combining the properties of bile salts and N-alkyl sulfobetaines, CHAPS exhibits superior ability to solubilize proteins without denaturation and is highly effective at disrupting protein-protein interactions.
Its small micellar molecular weight (~6,150 Da), low aggregation number (4–14), and high critical micelle concentration (6–10 mM) make it especially suitable for techniques requiring detergent removal by dialysis, such as protein purification, isoelectric focusing (IEF), and 2D electrophoresis.
Key Features & Benefits
- Zwitterionic Detergent – Combines features of both ionic and non-ionic surfactants
- Non-Denaturing Action – Preserves native protein structure during solubilization
- Superior Solubilization – More effective than sodium cholate or TRITON™ X-100 for membrane proteins
- Dialyzable – Easily removed from protein solutions or gels post-treatment
- Electrophoresis Compatible – Ideal for isoelectric focusing and 2D-PAGE applications
- Low Conductivity – <100 µmhos (1.0 M, 24 °C), suitable for sensitive biochemical workflows
Applications
- Membrane Protein Solubilization – Efficient for extracting functional membrane receptors and enzymes
- Protein-Protein Interaction Disruption – Used in dissociating complexes for analysis or purification
- Isoelectric Focusing (IEF) – Compatible with non-urea gels; provides high-resolution separation of proteins
- Two-Dimensional Electrophoresis (2D-PAGE) – Used in proteomics and subcellular protein analysis
- Receptor Studies – Solubilizes the opiate receptor with preserved ligand binding activity
- Cytochrome P450 Disaggregation – Converts P450 to its monomeric form without loss of function
Technical Specifications
| Attribute | Specification |
|---|---|
| Product Name | CHAPS, Molecular Biology Grade |
| Chemical Name | 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate |
| Molecular Weight | ~614.88 g/mol |
| Micellar Molecular Weight | ~6,150 Da |
| CMC | 6–10 mM (0.492–0.615% w/v) |
| Aggregation Number | 4–14 |
| Form | Powder |
| Conductivity | <100 µmhos (1.0 M, 24°C) |
| Detergent Type | Zwitterionic |
| Dialyzable | Yes |
| Storage Conditions | 2–8°C (following reconstitution); stable 6 months |
| Reagent Grade | Molecular Biology Grade |
| Toxicity | Harmful (GHS classification: C) |
| Regulatory Use | For Research Use Only |
Reconstitution & Storage
- Dissolve in buffer (e.g., water or IEF buffer) to desired concentration (commonly 2–4% w/v)
- Store reconstituted solution at 4°C; stable for up to 6 months
Conclusion
Sigma-Aldrich™ CHAPS, Molecular Biology Grade is a premium zwitterionic detergent for non-denaturing membrane protein isolation, protein complex dissociation, and electrophoretic analysis. Its dialyzability, superior solubilization profile, and compatibility with IEF and 2D-PAGE workflows make it an essential tool in proteomics, structural biology, and receptor biochemistry.
- Pack Size: 250g 1kg 1g 5g 10g 25g