0
Thermo Scientific™ Remel™ 2% Bile Solubility Reagent, 25mL
Catalog No :
CAS Number :
Brand :
In Stock
Liquid Reagent for Differentiation of Streptococcus pneumoniae
Specifications:
| Application | Clinical microbiology, Biochemical Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Biochemical Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Remel | ||
| Product Grade | Microbiology grade | ||
Thermo Scientific™ Remel™ 2% Bile Solubility Reagent is a ready-to-use liquid reagent formulated for the differentiation of Streptococcus pneumoniae from other alpha-hemolytic streptococci using either the Spot Test or Tube Test method.
The bile solubility test is a classical confirmatory procedure in clinical microbiology. S. pneumoniae is bile soluble and undergoes lysis when exposed to sodium desoxycholate, whereas other alpha-hemolytic streptococci remain unaffected. The 2% formulation provides a controlled and reliable alternative concentration for pneumococcal differentiation in diagnostic laboratories.
Principle of the Method
S. pneumoniae possesses an autolytic intracellular enzyme that lyses its own cell wall during cell division. When sodium desoxycholate is applied:
- Bile salts reduce surface tension at the medium–membrane interface.
- Pneumococcal cells become susceptible to membrane disruption.
- Visible lysis occurs in bile-soluble strains.
Other alpha-hemolytic streptococci do not undergo lysis under the same conditions.
Test Methods and Interpretation
Spot Test (Directly on Blood Agar)
Positive Result:
Colony disintegrates or dissolves within the recommended time frame.
Negative Result:
Colony remains intact.
Tube Test (Broth Suspension)
Positive Result:
Test suspension clears while the control suspension remains turbid.
Negative Result:
Both test and control suspensions remain turbid.
Product Specifications
| Parameter | Specification |
|---|---|
| Product Name | 2% Bile Solubility Reagent |
| Catalog Number | R21209-MBD (also known as R21209) |
| Format | Bottle |
| Quantity | 25 mL |
| Detectable Analyte | Streptococcus pneumoniae |
| Unit Size | Each |
Applications
- Clinical microbiology laboratories
- Pneumococcal confirmation testing
- Differentiation of alpha-hemolytic streptococci
- Hospital diagnostic workflows
- Support of bacterial identification protocols
Thermo Scientific Remel 2% Bile Solubility Reagent provides a reliable and standardized method for differentiating Streptococcus pneumoniae from other alpha-hemolytic streptococci. Suitable for both spot and tube test procedures, it supports accurate pneumococcal identification in clinical microbiology laboratories.