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Thermo Scientific Remel 10% Bile Solubility Reagent, 25mL
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Liquid Reagent for Differentiation of Streptococcus pneumoniae
Specifications:
| Application | Clinical microbiology, Biochemical Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Biochemical Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Remel | ||
| Product Grade | Microbiology grade | ||
Thermo Scientific™ Remel™ 10% Bile Solubility Reagent is a ready-to-use liquid reagent formulated for the differentiation of Streptococcus pneumoniae from other alpha-hemolytic streptococci using either the Spot Test or Tube Test method.
The bile solubility test is a classical confirmatory procedure in clinical microbiology. S. pneumoniae is bile soluble and undergoes rapid lysis when exposed to 10% sodium desoxycholate, while other alpha-hemolytic streptococci remain unaffected. This reagent provides reliable and rapid differentiation in both agar plate and broth-based testing formats.
Principle of the Method
S. pneumoniae possesses an autolytic intracellular enzyme that lyses its own cell wall during cell division. When 10% sodium desoxycholate is applied:
- The bile salts reduce surface tension at the medium–membrane interface.
- Pneumococcal cells become susceptible to membrane disruption.
- Visible lysis occurs in bile-soluble strains.
Other alpha-hemolytic streptococci do not undergo lysis under the same conditions.
Test Methods and Interpretation
Spot Test (Directly on Blood Agar)
Positive Result:
Colony disintegrates or dissolves within 30 minutes.
Negative Result:
Colony remains intact.
Tube Test (Broth Suspension)
Positive Result:
Test suspension clears within 3 hours; control suspension remains turbid.
Negative Result:
Both test and control suspensions remain turbid.
Reagent Composition
The 10% Bile Solubility Reagent contains:
- 10% w/v Sodium Desoxycholate
- Tincture Thimerosal
- Ethyl Alcohol
- Demineralized Water
Product Specifications
| Parameter | Specification |
|---|---|
| Product Name | 10% Bile Solubility Reagent |
| Catalog Number | R21206 |
| Format | Bottle |
| Quantity | 25 mL |
| Detectable Analyte | Streptococcus pneumoniae |
| Storage | Below 20°C |
| Unit Size | Each |
Applications
- Clinical microbiology laboratories
- Confirmation of pneumococcal isolates
- Differentiation of alpha-hemolytic streptococci
- Hospital diagnostic testing
- Support of bacterial identification workflows
Storage and Handling
Store below 20°C.
For professional laboratory use only.
Refer to official product documentation for complete instructions.
Thermo Scientific Remel 10% Bile Solubility Reagent provides a rapid, reliable method for differentiating Streptococcus pneumoniae from other alpha-hemolytic streptococci. Suitable for both spot and tube test procedures, it supports accurate identification in clinical microbiology laboratories and enhances diagnostic confidence.