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Thermo Scientific™ MgCl2 (magnesium chloride) (25 mM), 4 x 1.25 mL
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Specifications:
| Application | PCR | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ 25 mM MgCl₂ is a high-purity, PCR-grade solution of magnesium chloride optimized for use in DNA amplification and other enzymatic reactions. This reagent is membrane-filtered through a 0.22 μm filter, ensuring sterility and purity suitable for sensitive molecular biology protocols.
Magnesium ions play a crucial role in stabilizing primer-template interactions and activating DNA polymerases. This reagent is compatible with a wide range of polymerases, including:
- Taq DNA Polymerase (EP0072)
- DreamTaq DNA Polymerase (EP0701)
- Phire Hot Start II DNA Polymerase (F122L)
The formulation allows users to fine-tune magnesium ion concentration, a key factor influencing the specificity and yield of PCR reactions.
Key Features
- Optimized for PCR and Hot Start PCR: Enables improved reaction control and product fidelity
- Nuclease-free: Free from DNases, RNases, and phosphatases for maximal sample protection
- Convenient Packaging: Supplied as 4 × 1.25 mL aliquots to reduce contamination risk and ensure consistency
- Sterile Filtered: 0.22 µm membrane filtration ensures ultra-clean preparation
Specifications
| Attribute | Details |
|---|---|
| Chemical Name | Magnesium Chloride (MgCl₂) |
| Concentration | 25 mM |
| Volume | 4 × 1.25 mL |
| Form | Liquid |
| Application | Hot-start PCR, standard PCR, enzyme reactions |
| Purity | Free of nucleases and phosphatases |
| Storage | –20 °C (non-frost-free freezer) |
| Shipping Condition | Wet or Dry Ice |
Applications
- PCR optimization and master mix formulation
- Enzyme-based DNA/RNA amplification
- qPCR and RT-PCR setups
- Cloning and sequencing workflows
Thermo Scientific™ 25 mM MgCl₂ (R0971) is a premium-grade solution tailored for consistent, high-performance PCR and enzymatic reactions. Its nuclease-free formulation and compatibility with leading DNA polymerases make it an essential reagent for molecular biologists seeking precision and reproducibility. The convenient format and rigorous quality controls provide confidence in every reaction.