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Thermo Scientific™ FastDigest™ Value Pack, 13 x 20 μL reactions
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Enzymes; EcoR I, Bgl II, Nde I, Sal I, Xba I, Kpn I, Eco32I, Xho I, Not I, BamH I, Hind III, Sma I, Pst I
Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes and Substrates | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Comprehensive set of the 13 most commonly used FastDigest restriction enzymes
The Thermo Scientific™ FastDigest Value Pack contains a curated selection of 13 high-performance FastDigest enzymes, enabling a wide range of molecular biology applications including vector and insert preparation, fast clone analysis, and PCR product digestion. Each enzyme is supplied in a 20 μL format, providing enough for 20 standard reactions.
FastDigest enzymes deliver complete DNA digestion in just 5–15 minutes using a universal buffer system—eliminating the need for buffer changes or DNA purification before downstream steps. The FastDigest Green Buffer also contains density reagents and tracking dyes for direct gel loading, saving time and streamlining workflows.
Key Features & Benefits
- Complete Set – Includes 13 of the most frequently used restriction enzymes for cloning and analysis.
- Rapid Digestion – Achieve full digestion in 5–15 minutes.
- Universal Buffer System – All enzymes active in both FastDigest and FastDigest Green Buffers.
- Downstream Compatibility – No buffer changes required for ligations, PCR, or other enzymatic steps.
- Direct Gel Loading – FastDigest Green Buffer allows direct electrophoresis without extra handling.
- High Quality & Reliability – 100% activity and no star activity under recommended conditions.
Applications
- Molecular Cloning – Vector and insert preparation.
- Restriction Site Mapping – Analysis of DNA cut patterns.
- Clone Screening – Rapid digestion for verification.
- PCR Product Digestion – Fast processing for downstream workflows.
Enzymes Included
| Enzyme | Recognition Sequence | Overhang |
|---|---|---|
| EcoRI | G↓AATTC | Sticky 5′ |
| BglII | A↓GATCT | Sticky 5′ |
| NdeI | CA↓TATG | Sticky 5′ |
| SalI | G↓TCGAC | Sticky 5′ |
| XbaI | T↓CTAGA | Sticky 5′ |
| KpnI | GGTAC↓C | Sticky 3′ |
| Eco32I | GAT↓ATC | Blunt |
| XhoI | C↓TCGAG | Sticky 5′ |
| NotI | GC↓GGCCGC | Sticky 5′ |
| BamHI | G↓GATCC | Sticky 5′ |
| HindIII | A↓AGCTT | Sticky 5′ |
| SmaI | CCC↓GGG | Blunt |
| PstI | CTGCA↓G | Sticky 3′ |
Technical Specifications
| Property | Details |
|---|---|
| Product Type | Restriction Enzyme Value Pack |
| Quantity | 13 × 20 μL reactions |
| Enzymes Included | EcoRI, BglII, NdeI, SalI, XbaI, KpnI, Eco32I, XhoI, NotI, BamHI, HindIII, SmaI, PstI |
| Compatible Buffers | 10X FastDigest Buffer / 10X FastDigest Green Buffer |
| Reaction Time | 5–15 minutes |
| Overhangs | Mixed (sticky/blunt) |
| Type IIS Restriction Enzyme | No |
| Research Category | Traditional Cloning |
| Storage | –20°C |
| Star Activity | None under recommended conditions |
Kit Contents
- 13 FastDigest enzymes (20 μL each)
- 2 × 1.0 mL 10X FastDigest Buffer
- 2 × 1.0 mL 10X FastDigest Green Buffer
Ordering Information
| Catalog Number | Quantity |
|---|---|
| K1991 | 13 × 20 μL reactions |
The Thermo Scientific™ FastDigest Value Pack offers an all-in-one solution for laboratories performing routine and advanced molecular cloning workflows. By combining 13 of the most frequently used enzymes with a rapid, universal buffer system, it enables fast, efficient, and reproducible DNA digestion while minimizing workflow complexity. This pack is ideal for research groups seeking versatility, time savings, and high performance in one convenient kit.